波摩那钩端螺旋体的营养。III. 钙、镁和钾的需求
NUTRITION OF LEPTOSPIRA POMONA. III. CALCIUM, MAGNESIUM, AND POTASSIUM REQUIREMENTS.
作者信息
JOHNSON R C, GARY N D
出版信息
J Bacteriol. 1963 May;85(5):983-5. doi: 10.1128/jb.85.5.983-985.1963.
Johnson, R. C. (Fort Detrick, Frederick, Md.) and N. D. Gary. Nutrition of Leptospira pomona. III. Calcium, magnesium, and potassium requirements. J. Bacteriol. 85:983-985. 1963.-The cation requirements for Leptospira pomona Wickard were investigated employing a basal medium prepared with 10% deionized rabbit serum and containing 10(-3)m NH(4)Cl, 5 mug/ml thiamine, and 0.01 m Na(2)HPO(4) buffer (pH 7.4). Rabbit serum was deionized by passage through a column of Amberlite IRC-50 resin. This basal medium did not support growth when either Ca(++) or Mg(++) was added to it. However, when both Ca(++) (3 x 10(-5)m) and Mg(++) (5 x 10(-6)m) were present, growth was obtained. Although an absolute requirement for K(+) could not be demonstrated, an increase in the amount of growth did occur if K(+) (5 x 10(-5)m) was incorporated in addition to Ca(++) and Mg(++). When adequate concentrations of Ca(++) and K(+) were present with a limiting concentration of Mg(++) (less than 5 x 10(-7)m), filamentous cells were observed.
约翰逊，R.C.（马里兰州弗雷德里克市德特里克堡）和N.D.加里。波摩那钩端螺旋体的营养。III.钙、镁和钾的需求。《细菌学杂志》85:983 - 985。1963年。——采用一种基础培养基对波摩那钩端螺旋体威卡德株的阳离子需求进行了研究，该基础培养基由10%去离子兔血清制备而成，含有10⁻³m氯化铵、5微克/毫升硫胺素和0.01m磷酸氢二钠缓冲液（pH 7.4）。兔血清通过Amberlite IRC - 50树脂柱进行去离子处理。当向该基础培养基中添加Ca²⁺或Mg²⁺时，均不能支持其生长。然而，当同时存在Ca²⁺（3×10⁻⁵m）和Mg²⁺（5×10⁻⁶m）时，可实现生长。虽然无法证明对K⁺有绝对需求，但如果除了Ca²⁺和Mg²⁺之外还加入K⁺（5×10⁻⁵m），则生长量会增加。当存在足够浓度的Ca²⁺和K⁺以及有限浓度的Mg²⁺（小于5×10⁻⁷m）时，会观察到丝状细胞。
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