用于培养钩端螺旋体的无蛋白和低蛋白培养基。
Protein-free and low-protein media for the cultivation of Leptospira.
作者信息
Bey R F, Johnson R C
出版信息
Infect Immun. 1978 Feb;19(2):562-9. doi: 10.1128/iai.19.2.562-569.1978.
Abstract
A protein-free medium composed of charcoal-detoxified Tweens (polysorbates), vitamins B12 and B1, inorganic salts, and organic buffer is described that supports the growth and subculture of pathogenic and saprophytic Leptospira. Growth was initiated from small inocula, and cell densities of 10(9) organisms per ml were attained. Antigenicity and immunogenicity of Leptospira cultivated in this medium were similar to those of cells cultivated in serum-containing media. The protein-free medium was converted to a low-protein medium by the addition of 0.1% bovine serum albumin.
摘要
本文描述了一种无蛋白培养基，其由经活性炭解毒的吐温（聚山梨酯）、维生素B12和B1、无机盐以及有机缓冲剂组成，可支持致病性和腐生性钩端螺旋体的生长及传代培养。从少量接种物开始生长，可达到每毫升10(9)个生物体的细胞密度。在此培养基中培养的钩端螺旋体的抗原性和免疫原性与在含血清培养基中培养的细胞相似。通过添加0.1%的牛血清白蛋白，将无蛋白培养基转化为低蛋白培养基。
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2
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3
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