双氢链霉素对大肠杆菌中四氮唑染料还原的影响
EFFECT OF DIHYDROSTREPTOMYCIN ON TETRAZOLIUM DYE REDUCTION IN ESCHERICHIA COLI.
作者信息
BRAGG P D, POLGLASE W J
出版信息
J Bacteriol. 1963 Apr;85(4):795-800. doi: 10.1128/jb.85.4.795-800.1963.
Bragg, P. D. (University of British Columbia, Vancouver, British Columbia, Canada) and W. J. Polglase. Effect of dihydrostreptomycin on tetrazolium dye reduction in Escherichia coli. J. Bacteriol. 85:795-800. 1963.-Sonic-disrupted extracts of Escherichia coli, grown without added antibiotic (sensitive and resistant), contained (in supernatant of fraction centrifuged at 100,000 x g) a dihydrostreptomycin-inhibitable, succinate-triphenyltetrazolium chloride (TTC) reductase activity. The succinate-TTC reductase activities of extracts of E. coli grown in the presence of dihydrostreptomycin (resistant and dependent) were relatively low and were not inhibited by the antibiotic. At a moderate magnesium concentration, the degree of inhibition by dihydrostreptomycin of succinate-TTC reductase activity was sufficiently marked to indicate an important site of action of the antibiotic. Magnesium, putrescine, and spermidine antagonized the action of dihydrostreptomycin in the succinate-TTC reductase system.
布拉格，P.D.（加拿大不列颠哥伦比亚省温哥华市英属哥伦比亚大学）和W.J.波尔格拉斯。双氢链霉素对大肠杆菌中四氮唑染料还原的影响。《细菌学杂志》85:795 - 800。1963年。——在不添加抗生素的情况下培养的大肠杆菌（敏感型和抗性型）的超声破碎提取物（在100,000×g离心后的上清液中）含有一种可被双氢链霉素抑制的琥珀酸 - 氯化三苯基四氮唑（TTC）还原酶活性。在双氢链霉素存在下培养的大肠杆菌（抗性型和依赖型）提取物的琥珀酸 - TTC还原酶活性相对较低，且不受该抗生素抑制。在中等镁浓度下，双氢链霉素对琥珀酸 - TTC还原酶活性的抑制程度足够显著，表明该抗生素的一个重要作用位点。镁、腐胺和亚精胺在琥珀酸 - TTC还原酶系统中拮抗双氢链霉素的作用。
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