Lyamuya E F, Nyanda P, Mohammedali H, Mhalu F S
Department of Microbiology and Immunology, Muhimbili University College of Health Sciences, Dar es Salaam, Tanzania.
J Trop Med Hyg. 1992 Oct;95(5):335-8.
Thirty-one specimens from patients involved in an outbreak of plague were cultured. Suspicious isolates were presumptively identified by colonial characteristics, simple strains and by API 20E, and confirmed by inoculation into white mice. Antimicrobial susceptibility testing was also done. The isolation rate of Y. pestis was 22.6%. All isolates were uniformly sensitive to tetracycline, streptomycin, sulphadimidine and chloramphenicol; moderately sensitive to erythromycin, and resistant to trimethoprim. Y. pestis was confirmed as a cause of the epidemic and sensitivity of Y. pestis to the four antimicrobial agents used was demonstrated. It is recommended that laboratories in zonal hospitals serving areas with plague foci should be provided with facilities for isolation and preliminary identification of Y. pestis in order to speed up diagnosis of plague outbreaks. Confirmation of the identity, biotyping and antibiotic susceptibility testing should be undertaken in a well established national reference laboratory. This appears to be the first publication on bacteriological investigations of human plague in Tanzania.
对31份来自鼠疫暴发疫情患者的样本进行了培养。通过菌落特征、简单菌株鉴定以及API 20E对可疑分离株进行初步鉴定，并通过接种到小白鼠体内进行确认。同时还进行了药敏试验。鼠疫耶尔森菌的分离率为22.6%。所有分离株对四环素、链霉素、磺胺二甲嘧啶和氯霉素均呈一致敏感性；对红霉素中度敏感，对甲氧苄啶耐药。证实了鼠疫耶尔森菌是此次疫情的病因，并证明了鼠疫耶尔森菌对所使用的四种抗菌药物的敏感性。建议为服务于鼠疫疫源地地区的地区医院实验室配备鼠疫耶尔森菌分离和初步鉴定设施，以加快鼠疫暴发疫情的诊断。应在设备完善的国家参考实验室进行菌种鉴定、生物分型和抗生素药敏试验。这似乎是坦桑尼亚关于人类鼠疫细菌学调查的首次出版物。
