Endo J, Notsu Y
Dept. of Laboratory Medicine, Shimane Medical University.
Nihon Rinsho. 1992 Jul;50(7):1509-15.
Recent progress in the field of amino-acid analysis, characterized by the development of fully automated chromatographic equipment, is described. For separation and quantitation of free amino acids from biological fluid, such as blood and urine, ion exchange column chromatography followed by colorimetric detection of ninhydrin reactive products is the most widely adopted methodology in the clinical field. Analytic procedures are fully automated. The computer controlled pumping mechanism guarantees a precise buffer supply for separation and peak identification and peak height or/and area detection of each amino acid in the effluent are highly reproducible. Complete analysis of more than 30 amino acids in biological fluids can be performed within 2 hours. Amino acid analysis is now easily adopted in the routine clinical laboratory and is expected to contribute to the clinical diagnosis of amino acidopathies.
本文描述了氨基酸分析领域的最新进展，其特点是全自动色谱设备的发展。对于从血液和尿液等生物流体中分离和定量游离氨基酸，离子交换柱色谱法随后对茚三酮反应产物进行比色检测是临床领域最广泛采用的方法。分析过程完全自动化。计算机控制的泵送机制确保为分离提供精确的缓冲液供应，并且流出物中每种氨基酸的峰识别以及峰高或/和面积检测具有高度的可重复性。生物流体中30多种氨基酸的完整分析可在2小时内完成。氨基酸分析现在很容易在常规临床实验室中采用，并有望有助于氨基酸病的临床诊断。
