死后血样中糖化白蛋白作为糖尿病诊断参数的分析

Analysis of glycated albumin in postmortem blood samples as the diagnostic parameters of diabetes mellitus.

作者信息

Akane A, Shiono H, Matsubara K, Tanabe K, Nakamura H, Hasegawa M, Kagawa M

机构信息

Department of Legal Medicine, Shimane Medical University, Izumo, Japan.

出版信息

Nihon Hoigaku Zasshi. 1992 Aug;46(4):237-43.

PMID:1405016

Abstract

Glycated hemoglobin (GHb), fructosamine and glycated albumin (GA) in hemolytic sera from cadavers were analyzed for the postmortem diagnosis of diabetes mellitus. The levels of GHb and fructosamine were determined by boronate affinity chromatography and colorimetry, respectively. Albumin fraction was isolated from the samples by Affi-Gel Blue affinity chromatography. The glycated and non-glycated molecules were separated by boronate affinity chromatography, and quantitated by bromcresol green method. Fructosamine could not be analyzed from highly hemolytic sera containing more than 10 g/l hemoglobin. In such samples, the levels of GHb and GA were deviated from the standard values, indicating their postmortem degradation. In less hemolytic samples, GA was as informative as GHb and fructosamine for the diagnosis of diabetes mellitus.

摘要

对尸体溶血血清中的糖化血红蛋白（GHb）、果糖胺和糖化白蛋白（GA）进行分析，用于糖尿病的死后诊断。GHb和果糖胺的水平分别通过硼酸亲和色谱法和比色法测定。通过Affi-Gel Blue亲和色谱法从样品中分离白蛋白组分。糖化和非糖化分子通过硼酸亲和色谱法分离，并通过溴甲酚绿法进行定量。对于血红蛋白含量超过10 g/l的高度溶血血清，无法分析果糖胺。在这类样品中，GHb和GA的水平偏离标准值，表明它们在死后发生了降解。在溶血程度较轻的样品中，GA对于糖尿病诊断的信息量与GHb和果糖胺相当。