Kapel'ko V I, Kupriianov V V, Novikova N A, Lakomkin V L, Shteĭnshneĭder A Ia, Korchazhkina O V, Ruuge E K, Saks V A
Kardiologiia. 1992 Apr;32(4):71-4.
To study the relative roles of creatine kinase (CK) and adenylate systems in cardiac energy turnover, the effect of CK inhibitor, iodoacetamide- (IAA, 0.5 mM), and 2-deoxyglucose-(DOG, 2 mM) induced) 65% depletion of adenine nucleotides at slightly decreased CK flux was determined in isolated rat heart. Both substances did not substantially affect contractile parameters of the isovolumic heart. However, an augmentation of cardiac work induced by isoproterenol addition was feeble and transient in IAA-treated hearts while the response of DOG-treated hearts was well preserved. The cardiac failure after IAA treatment was associated with irreversible fall in myocardial ATP content as evidenced by 31P-NMR technique. Furthermore, these hearts were unable to perform cardiac pump function due to insufficient cardiac filling and distensibility. The DOG-treated hearts exhibited 50% reduction in the pump function and were able to increase their work in elevated resistance. The results suggest that CK pathway is extremely important for both full cardiac relaxation and maximal contractile function.
为研究肌酸激酶（CK）和腺苷酸系统在心脏能量转换中的相对作用，在离体大鼠心脏中测定了CK抑制剂碘乙酰胺（IAA，0.5 mM）和2-脱氧葡萄糖（DOG，2 mM）诱导的腺嘌呤核苷酸65%耗竭对CK通量略有降低的影响。两种物质均未显著影响等容心脏的收缩参数。然而，在IAA处理的心脏中，异丙肾上腺素诱导的心脏作功增强微弱且短暂，而DOG处理的心脏的反应则得到很好的保留。IAA处理后的心力衰竭与心肌ATP含量的不可逆下降有关，这通过31P-NMR技术得以证实。此外，这些心脏由于心脏充盈不足和扩张性不足而无法执行心脏泵功能。DOG处理的心脏泵功能降低了50%，并能够在升高的阻力下增加其作功。结果表明，CK途径对于心脏的完全舒张和最大收缩功能都极为重要。
