Alpers C E, Seifert R A, Hudkins K L, Johnson R J, Bowen-Pope D F
Department of Pathology, University of Washington School of Medicine, Seattle.
Kidney Int. 1992 Aug;42(2):390-9. doi: 10.1038/ki.1992.300.
Expression of PDGF B-chain and the PDGF receptor beta-subunit (PDGFR beta) is detected immunocytochemically during the development of glomeruli in human kidneys of 54 to 105 days gestational age. During the early stages (vesicular, comma-shape and S-shape) of glomerulogenesis, PDGF B-chain is localized to differentiating epithelium of the glomerular vesicle, while PDGFR beta is expressed in the undifferentiated metanephric blastema, vascular structures, and interstitial cells. During this stage PDGF may be acting as a paracrine growth factor and as a chemoattractant acting to recruit mesangial progenitor cells into the developing glomerulus. As the glomerular tuft forms, both PDGF B-chain and PDGFR beta can be detected in an arboreal pattern radiating from the hilus of the glomerular tuft. Immunocytochemical studies using markers specific to endothelium (Ulex europaeus I lectin, Factor VIII related antigen), and smooth muscle (alpha-smooth muscle actin), indicate that the PDGF B-chain and PDGFR beta are both expressed primarily by mesangial cells. During this stage, PDGF may be acting primarily to provide an autocrine factor to mediate further mesangial cell proliferation. Glomerular expression of alpha-smooth muscle actin is limited to later stages of glomerulogenesis; at these stages the pattern of expression is similar to that of PDGF-B chain and PDGFR beta. The upregulation of mesangial PDGF, PDGFR beta, and alpha-smooth muscle actin expression that has been identified in some disease states in both humans and experimental animals appears to represent a recapitulation of this normal developmental process.
在孕龄54至105天的人肾肾小球发育过程中，通过免疫细胞化学检测到血小板衍生生长因子B链（PDGF B链）和血小板衍生生长因子受体β亚基（PDGFRβ）的表达。在肾小球发生的早期阶段（囊泡期、逗号形期和S形期），PDGF B链定位于肾小球囊泡的分化上皮，而PDGFRβ在未分化的后肾胚基、血管结构和间质细胞中表达。在此阶段，PDGF可能作为旁分泌生长因子和趋化因子，作用是将系膜祖细胞招募到发育中的肾小球。随着肾小球毛细血管丛形成，在从肾小球毛细血管丛的肾门呈树状放射状分布的区域可检测到PDGF B链和PDGFRβ。使用内皮特异性标志物（欧洲荆豆I凝集素、VIII因子相关抗原）和平滑肌特异性标志物（α平滑肌肌动蛋白）进行的免疫细胞化学研究表明，PDGF B链和PDGFRβ主要由系膜细胞表达。在此阶段，PDGF可能主要作为自分泌因子，介导系膜细胞的进一步增殖。α平滑肌肌动蛋白的肾小球表达仅限于肾小球发生的后期阶段；在这些阶段，其表达模式与PDGF - B链和PDGFRβ相似。在人类和实验动物的某些疾病状态中已确定的系膜PDGF、PDGFRβ和α平滑肌肌动蛋白表达上调，似乎代表了这一正常发育过程的重现。
