Beta protein precursor expression in human platelets and a megakaryocyte cell line. Possible implications for the origin of cerebral amyloidosis in Alzheimer's disease.

BACKGROUND

The origin of the amyloid beta protein (A beta) that is the main constituent of amyloid fibrils occurring in the senile plaques and cerebrovasculature of individuals afflicted with Alzheimer's Disease, Down's Syndrome, Hereditary Cerebral Hemorrhage with Amyloidosis--Dutch Type, and Sporadic Cerebral Amyloid Angiopathy, is central to the pathogenesis of these disorders. Evidence exists to support a neuronal and/or a vascular origin. We have reported that platelets may serve as one possible source of the A beta sequence via an intact, membrane-associated Beta Protein Precursor (beta PP) which is encoded by a platelet transcript (BBRC 173:1292-1298, 1990).

EXPERIMENTAL DESIGN

Immunoaffinity chromatography and western blotting of extracted cellular proteins, polymerase chain reaction amplification of beta PP mRNA, fluorescence activated flow cytometric analysis, and confocal scanning laser microscopy have been employed to characterize the presence and distribution of thrombocytic beta PP.

RESULTS

Immunoblot analysis with antibodies specific for the carboxyl-terminal end of beta PP indicates that platelets and the Dami megakaryocyte cell line express membrane-associated species of intact beta PP ranging in molecular weight from 110 to 140 kilodaltons (kd), as well as carboxyl-terminal reactive forms ranging from 16 to 22 kd. Thrombin stimulation of platelets induces the release of five soluble beta PP species, which possess apparent isofocusing points in the range of 4.1-5.5. By contrast, extracts of peripheral blood mononuclear cells enriched by ficoll centrifugation, endothelial cells and a B cell line were not immunoreactive by western blot, even though beta PP transcripts could be amplified by polymerase chain reaction. The distribution of platelet beta PP was localized by flow cytometric analysis and scanning laser microscopy, using fluorescein-labeled antibodies. Study of the subcellular distribution of platelet beta PP indicates that these translation products are accumulated in discrete foci throughout the thrombocyte, possibly corresponding to secretory granules.

CONCLUSIONS

The size of the carboxyl-terminal forms of beta PP indicate that the A beta sequence is present as a membrane associated constituent in unstimulated platelets, and may represent alternative pathways of beta PP processing. Cleavage or other abnormal processing of platelet-associated beta PP in Alzheimer's disease provides one mechanism whereby cerebral amyloid might derive from the circulation.