Verbeek M M, Otte-Höller I, Wesseling P, Van Nostrand W E, Sorg C, Ruiter D J, de Waal R M
Department of Pathology, University Hospital Nijmegen, The Netherlands.
Acta Neuropathol. 1995;90(5):493-503. doi: 10.1007/BF00294811.
One of the major histopathological lesions in brains of patients with dementia of the Alzheimer type (DAT) is the senile plaque. Although previous studies have shown that senile plaques are often accompanied by microglial cells, the role of these cells in DAT pathology is still unclear. In an immunohistochemical and immunoelectron microscopical analysis of DAT and control brain tissues we addressed this issue using two monoclonal antibodies (mAbs KP1 and 25F9) directed against lysosomal antigens in monocytes and macrophages. Whereas KP1 stained lysosomes in both resting and activated microglial cells, 25F9-staining was predominantly found in lysosomes of activated microglial cells in classic senile plaques. The number and size of 25F9-positive lysosomes in activated microglial cells was increased compared to 25F9-staining in unaffected areas in DAT and control sections. We conclude that mAb 25F9 is a unique and useful lysosomal marker, with a higher specificity than other known markers, for activated microglial cells associated with classic, but not with diffuse, senile plaques.
阿尔茨海默型痴呆（DAT）患者大脑中的主要组织病理学病变之一是老年斑。尽管先前的研究表明老年斑常伴有小胶质细胞，但这些细胞在DAT病理学中的作用仍不清楚。在对DAT和对照脑组织进行的免疫组织化学和免疫电子显微镜分析中，我们使用两种针对单核细胞和巨噬细胞溶酶体抗原的单克隆抗体（mAb KP1和25F9）来解决这个问题。KP1可对静息和活化的小胶质细胞中的溶酶体进行染色，而25F9染色主要见于典型老年斑中活化小胶质细胞的溶酶体。与DAT和对照切片中未受影响区域的25F9染色相比，活化小胶质细胞中25F9阳性溶酶体的数量和大小有所增加。我们得出结论，mAb 25F9是一种独特且有用的溶酶体标记物，对于与典型而非弥漫性老年斑相关的活化小胶质细胞，其特异性高于其他已知标记物。
