Imdahl A, Kisker O, Kirste G, Farthmann E H
Chirurgische Universitätsklinik Freiburg, Abteilung Allgemeine Chirurgie mit Poliklinik.
J Surg Res. 1992 Aug;53(2):123-7. doi: 10.1016/0022-4804(92)90023-s.
The terminal glycoproteins of fetal, cultivated (7-12 days), and adult nondiabetic and diabetic pancreatic tissues (Balb c, C3h mice) were investigated by lectin histology (peanut-, phytohemagglutinin, wheat germ agglutinin, Ulex europeus I, concanavalin A and Ricinus communis agglutinin, PaP method +/- neuraminidase). Anti-insulin and -glucagon were used to identify islet cells. S-100 antibody showed dendritic reticulum cells, anti-IAK proved MHC II antigens (C3h). Cultured tissue was partly incubated with anti-IAK and complement for lysis of MHC II antigens. On the 19th gestational day fetal pancreatic tissue did not bind peanut agglutinin, Phytohemagglutinin, or wheat germ agglutinin, whereas concanavalin A and Ricinus communis were weakly bound. Terminal fucose residues were not expressed by C3h fetal islet cells in contrast to Balb c. Following neuraminidase digestion peanut agglutinin and phytohemagglutinin were strongly bound, indicating sialic acid-substituted terminal glycoproteins. Cultivated tissue (Day 7) bound all investigated lectins (except Ulex europeus I in C3h mice), indicating maturation of islet cells. In spite of the peak of insulin concentration in the medium we observed a faint binding of anti-insulin and investigated lectins following 12 days of cultivation. This indicates a disorder of terminal glycoprotein synthesis at this point. There was no difference in lectin binding patterns of adult nondiabetic islet cells compared to the cultivated tissue (7 days), but no Ulex europaeus I binding of the adult Balb c mice was observed. S-100 binding decreased during the cultivation period as dendritic reticulum cells became destroyed by cultivation.(ABSTRACT TRUNCATED AT 250 WORDS)
通过凝集素组织学（花生凝集素、植物血凝素、麦胚凝集素、荆豆凝集素I、刀豆球蛋白A和蓖麻凝集素，过碘酸盐-赖氨酸-多聚甲醛法±神经氨酸酶）研究了胎儿、培养（7 - 12天）以及成年非糖尿病和糖尿病胰腺组织（Balb c、C3h小鼠）的终末糖蛋白。使用抗胰岛素和抗胰高血糖素抗体鉴定胰岛细胞。S - 100抗体显示树突状网状细胞，抗IAK证明MHC II抗原（C3h）。将培养的组织部分与抗IAK和补体一起孵育以裂解MHC II抗原。在妊娠第19天，胎儿胰腺组织不结合花生凝集素、植物血凝素或麦胚凝集素，而刀豆球蛋白A和蓖麻凝集素结合较弱。与Balb c不同，C3h胎儿胰岛细胞不表达终末岩藻糖残基。经神经氨酸酶消化后，花生凝集素和植物血凝素强烈结合，表明存在唾液酸取代的终末糖蛋白。培养组织（第7天）结合所有研究的凝集素（C3h小鼠中的荆豆凝集素I除外），表明胰岛细胞成熟。尽管培养基中胰岛素浓度达到峰值，但培养12天后我们观察到抗胰岛素和研究的凝集素结合较弱。这表明此时终末糖蛋白合成出现紊乱。成年非糖尿病胰岛细胞的凝集素结合模式与培养组织（7天）相比没有差异，但未观察到成年Balb c小鼠结合荆豆凝集素I。随着培养过程中树突状网状细胞被破坏，S - 100结合减少。（摘要截短至250字）
