KWAPINSKI J B
J Bacteriol. 1963 Aug;86(2):179-86. doi: 10.1128/jb.86.2.179-186.1963.
Kwapinski, J. B. (University of New England, Armidale, N.S.W., Australia). Antigenic structure of Actinomycetales. VI. Serological relationships between antigenic fractions of Actinomyces and Nocardia. J. Bacteriol. 86:179-186. 1963.-A total of 52 chemical fractions were obtained by a comprehensive technique of preparation from three strains of Actinomyces and three strains of Nocardia. The chemical and serological structures and specificities of disintegrated cells, cell walls, cytoplasms, and individual fractions were thoroughly studied. Cytoplasmic materials were found to be serologically alike or identical. The polysaccharide fractions, extracted from cell walls with alkali, formamide, and phenol, proved to be serologically related. Fractions prepared from the Nocardia by extractions in hot and concentrated solutions of acetic acid and sodium hydroxide, as well as the second protein fraction and the acetate-extracted polysaccharides of both the Nocardia and Actinomyces, proved to be genus-specific.
夸平斯基,J. B.(澳大利亚新南威尔士州阿米代尔新英格兰大学)。放线菌目抗原结构。VI. 放线菌属和诺卡氏菌属抗原组分之间的血清学关系。《细菌学杂志》86:179 - 186。1963年。——通过一种综合制备技术从三株放线菌和三株诺卡氏菌中总共获得了52种化学组分。对破碎细胞、细胞壁、细胞质和各个组分的化学和血清学结构及特异性进行了深入研究。发现细胞质物质在血清学上相似或相同。用碱、甲酰胺和苯酚从细胞壁中提取的多糖组分在血清学上被证明是相关的。通过在热的浓乙酸和氢氧化钠溶液中提取从诺卡氏菌制备的组分,以及诺卡氏菌和放线菌的第二种蛋白质组分和乙酸盐提取的多糖,被证明是属特异性的。