ABOOD L G, KOYAMA I
Science. 1963 Sep 27;141(3587):1277-8. doi: 10.1126/science.141.3587.1277.
In the presence of Ringer's solution containing a high concentration of potassium ions, the incorporation of P(32)-orthophosphate into adenosine triphosphate and other organophosphates of the desheathed sciatic nerve of the bullfrog was inhibited by more than 75 percent. In calcium-free Ringer's solution containing ethylenediaminetetraacetic acid, the inhibition was about 30 percent. Both solutions depolarized the nerves, and the depolarization probably interfered with the mechanisms of phosphate transfer rather than directly with phosphorylation. The inhibition may have been a consequence of the decreased adsorption of phosphates which occurs at the excitable surface during depolarization.
在含有高浓度钾离子的林格氏液中，牛蛙去鞘坐骨神经中P(32)-正磷酸盐掺入三磷酸腺苷和其他有机磷酸盐的过程受到超过75%的抑制。在含有乙二胺四乙酸的无钙林格氏液中，抑制率约为30%。两种溶液都会使神经去极化，而去极化可能干扰了磷酸盐转移机制，而非直接影响磷酸化作用。这种抑制可能是去极化过程中在可兴奋表面发生的磷酸盐吸附减少的结果。
