DURHAM N N
J Bacteriol. 1963 Sep;86(3):380-6. doi: 10.1128/jb.86.3.380-386.1963.
Durham, Norman N. (Oklahoma State University, Stillwater). Inhibition of microbial growth and separation by d-serine, vancomycin, and mitomycin C. J. Bacteriol. 86:380-386. 1963.-A study was made of the growth and separation inhibition of Pseudomonas fluorescens by vancomycin and mitomycin C and of a Flavobacterium sp. sensitive to mitomycin C and d-serine. Aberrant morphological forms (primarily "boat-shaped" cells and "chain-like" filaments) were observed during growth of the sensitive organism in the presence of the inhibitors. Reversal studies indicated that Mg(++), and to a lesser extent Mn(++), partially reversed vancomycin and mitomycin C growth inhibition during the early stages of growth but did not influence d-serine inhibition. Selected metabolites also showed a tendency to partially reverse mitomycin C and vancomycin inhibition of growth during the initial growth phase, and the differences in reversal trends suggested that d-serine, vancomycin, and mitomycin C produce metabolic lesions at different sites in the cell with respect to growth. Cultivation of the sensitive organism in low concentrations of the inhibitors resulted in a structurally fragile population, indicating formation of a defective cell structure. Mg(++) partially reversed the vancomycin inhibition of separation in these organisms. None of the other metals or metabolites, either singly or in varied combinations, was able to reverse inhibition of separation by the various inhibitors.
达勒姆，诺曼·N.（俄克拉荷马州立大学，斯蒂尔沃特）。D-丝氨酸、万古霉素和丝裂霉素C对微生物生长的抑制及分离作用。《细菌学杂志》86:380 - 386。1963年。——研究了万古霉素和丝裂霉素C对荧光假单胞菌生长和分离的抑制作用，以及一种对丝裂霉素C和D-丝氨酸敏感的黄杆菌属细菌。在敏感菌于抑制剂存在下生长过程中，观察到了异常形态（主要是“船形”细胞和“链状”丝状体）。逆转研究表明，Mg(++)，在较小程度上还有Mn(++)，在生长早期部分逆转了万古霉素和丝裂霉素C对生长的抑制作用，但不影响D-丝氨酸的抑制作用。选定的代谢物在初始生长阶段也显示出部分逆转丝裂霉素C和万古霉素对生长抑制的趋势，且逆转趋势的差异表明，D-丝氨酸、万古霉素和丝裂霉素C在细胞内关于生长的不同位点产生代谢损伤。在低浓度抑制剂中培养敏感菌会导致群体结构脆弱，表明形成了有缺陷的细胞结构。Mg(++)部分逆转了这些生物体中万古霉素对分离的抑制作用。其他金属或代谢物，无论是单独还是以不同组合，均无法逆转各种抑制剂对分离的抑制作用。
