奇异变形杆菌中大肠杆菌素K的性质。
The nature of colicin K from Proteus mirabilis.
作者信息
Jesaitis M A
出版信息
J Exp Med. 1970 May 1;131(5):1016-38. doi: 10.1084/jem.131.5.1016.
The colicinogenic factor K has been transferred from E. coli K 235 to Proteus mirabilis. The DNA of the colicinogenic Proteus has been shown to contain a small amount of a satellite DNA which presumably harbors the Col K factor. In the presence of mitomycin C the colicinogenic Proteus secretes colicin K into the growth medium. The bacteriocin has been purified by chromatography and obtained as an immunologically homogeneous substance unconjugated with other antigens of the Proteus bacillus. Proteus colicin K is a protein of relatively low molecular weight. It contains all of the usual amino acids except cysteine and is free of lipids and polysaccharides. The bacteriocin can be separated by electrofocusing into two major components. The latter have the same biological properties but differ in their specific electrical charges.
产大肠杆菌素因子K已从大肠杆菌K235转移至奇异变形杆菌。已证明产大肠杆菌素的变形杆菌的DNA含有少量卫星DNA，推测该卫星DNA携带Col K因子。在丝裂霉素C存在的情况下，产大肠杆菌素的变形杆菌将大肠杆菌素K分泌到生长培养基中。该细菌素已通过色谱法纯化，并获得了一种与变形杆菌的其他抗原未结合的免疫均质物质。变形杆菌大肠杆菌素K是一种分子量相对较低的蛋白质。它包含除半胱氨酸以外的所有常见氨基酸，且不含脂质和多糖。该细菌素可通过电聚焦分离为两个主要成分。后者具有相同的生物学特性，但比电荷不同。
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