BATES J L, LIU P V
J Bacteriol. 1963 Sep;86(3):585-92. doi: 10.1128/jb.86.3.585-592.1963.
Bates, Janice L. (University of Louisville School of Medicine, Louisville, Ky.) and Pinghui V. Liu. Complementation of lecithinase activities by closely related pseudomonads: its taxonomic implication. J. Bacteriol. 86:585-592. 1963.-A lecithinase activity was demonstrated by a mixture of cell-free extracts from two strains of closely related pseudomonads, neither of which was able to show this activity when grown alone. One component, from B-2265 (Pseudomonas aureofaciens var. nonliquefaciens), was heatlabile, nondialyzable, and antigenic. The other component, from 22/3 (P. fluorescens), was relatively heat-stable, nondialyzable, and nonantigenic. Antisera produced with the component of B-2265 alone neutralized the activity of the complete enzyme made up with both components, as well as that of similar enzymes of related species, such as P. aureofaciens, P. chlororaphis, and P. fluorescens. The taxonomic implication of these findings is discussed. The suggestion is made that determination of the serological specificity of an enzyme or its antigenic subunits is a more reliable criterion for establishing relationships of bacterial strains than methods depending on the overall similarity of biochemical activities.
贝茨，贾尼斯·L.（肯塔基州路易斯维尔市路易斯维尔大学医学院）和平辉·V.刘。密切相关的假单胞菌对卵磷脂酶活性的互补作用：其分类学意义。《细菌学杂志》86:585 - 592。1963年。——两种密切相关的假单胞菌菌株的无细胞提取物混合物表现出卵磷脂酶活性，而单独培养时这两种菌株均未表现出这种活性。一种成分来自B - 2265（非液化金色假单胞菌），对热不稳定，不可透析，且具有抗原性。另一种成分来自22/3（荧光假单胞菌），相对耐热，不可透析，且无抗原性。仅用B - 2265的成分产生的抗血清中和了由两种成分组成的完整酶的活性，以及相关物种如金色假单胞菌、绿针假单胞菌和荧光假单胞菌的类似酶的活性。讨论了这些发现的分类学意义。有人提出，确定一种酶或其抗原亚基的血清学特异性比依赖生化活性总体相似性的方法是建立细菌菌株关系更可靠的标准。
