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[流感病毒基本结构蛋白的制备性分离]

[Preparative isolation of basic structural proteins of the influenza virus].

作者信息

Ksenofontov A L, Fedorova N V, Glushakova S E

出版信息

Mol Gen Mikrobiol Virusol. 1992 Mar-Apr(3-4):3-6.

PMID:1406760
Abstract

The schemes for preparative electrophoretic isolation and purification of major proteins from influenza virus are described. The viral envelope protein, hemagglutinin, two of its subunits, internal M and NP proteins of influenza viruses A/FPV/Rostock (H7N1), A/PR/8/34 (H1N1) and X-31 (H3N2) were obtained in preparative amounts and characterized by amino acid and N-terminus analyses.

摘要

本文描述了从流感病毒中制备性电泳分离和纯化主要蛋白质的方案。获得了甲型流感病毒A/FPV/Rostock(H7N1)、A/PR/8/34(H1N1)和X-31(H3N2)的病毒包膜蛋白血凝素及其两个亚基、内部M蛋白和NP蛋白的制备量,并通过氨基酸分析和N端分析对其进行了表征。

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