CHANCE B, PARSONS D F
Science. 1963 Nov 29;142(3596):1176-80. doi: 10.1126/science.142.3596.1176.
Projecting subunits of an average diameter of 80 A are found on the cristae of mitochondria prepared from the muscle of Ascaris lumbricoides. A spectroscopic examination of the cytochrome content of these mitochondria shows no detectable cytochrome c(1), a, or a(3) and does reveal cytochromes of types c and b. Subunits in the same size range are found in cytochrome c deficient mitochondria of the emergent bee, while the frequency of their occurrence along the cristae is decreased relative to the adult bee. Apparently, the cytochrome content of the respiratory chain is not related to the size of the subunits, but may be related to the frequency of occurrence of the subunits.
在从蛔虫肌肉制备的线粒体嵴上发现了平均直径为80埃的突出亚基。对这些线粒体的细胞色素含量进行光谱检查发现,未检测到细胞色素c(1)、a或a(3),但确实发现了c型和b型细胞色素。在新出现的蜜蜂的缺乏细胞色素c的线粒体中也发现了相同大小范围的亚基,但其沿嵴出现的频率相对于成年蜜蜂有所降低。显然,呼吸链的细胞色素含量与亚基大小无关,但可能与亚基出现的频率有关。