BENDICH A, BORENFREUND E, ITTENSOHN O L, HUTCHISON D J
J Cell Biol. 1963 Oct;19(1):71-7. doi: 10.1083/jcb.19.1.71.
Chromosomes isolated from mouse leukemia L1210 cells were taken up by mouse macrophages, HeLa cells, and rat embryo fibroblasts following simple exposure in vitro. The process, which resembles pinocytosis or phagocytosis, was traced by autoradiography of chromosomes prelabeled with thymidine-H(3), and by staining techniques and phase contrast microscopy. During the first six hours, the uptake of chromosomes was restricted to the cytoplasm, but there was some evidence of penetration into the nucleus after 16 and 26 hours of exposure. Treatment of rat fibroblasts with glucose and insulin markedly enhanced the uptake of chromosomes, whereas iodoacetate inhibited their penetration.
从小鼠白血病L1210细胞中分离出的染色体,在体外简单暴露后被小鼠巨噬细胞、HeLa细胞和大鼠胚胎成纤维细胞摄取。这个类似于胞饮作用或吞噬作用的过程,通过用胸苷-H(3)预标记的染色体的放射自显影、染色技术和相差显微镜进行追踪。在最初的六个小时内,染色体的摄取仅限于细胞质,但在暴露16小时和26小时后,有一些证据表明染色体穿透进入了细胞核。用葡萄糖和胰岛素处理大鼠成纤维细胞显著增强了染色体的摄取,而碘乙酸则抑制了它们的穿透。