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用[3H]胸腺嘧啶核苷和[125I]碘脱氧尿苷进行双重标记作为一种在体内确定注射的DNA和细胞命运的方法。

Double labeling with [3H]thymidine and [125I]iododeoxyuridine as a method for determining the fate of injected DNA and cells in vivo.

作者信息

Myers D K, Feinendegen L E

出版信息

J Cell Biol. 1975 Nov;67(2PT.1):484-8. doi: 10.1083/jcb.67.2.484.

DOI:10.1083/jcb.67.2.484
PMID:1194358
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2109598/
Abstract

Mice were injected intravenously and intraperitoneally with preparations of intestinal nucleoprotein, spleen nuclei, mouse thymus cells, or human kidney T cells whose DNA had been labeled with both [3H]thymidine (TdR) and [125I]-iododeoxyuridine (IUdR). Since free TdR is reutilized more efficiently than free IUdR produced by enzymic hydrolysis of the exogenous DNA, the ratio of [3H]TdR/[125I]IUdR in the DNA fraction of the tissues of the recipient mice provides a measure of the amount of intact exogenous DNA in the tissue. In most instances, the doubly labeled exogenous DNA was almost completely hydrolyzed within 1 day injection, but survival of the DNA from whole cells could be demonstrated in some cases.

摘要

给小鼠静脉内和腹腔内注射经[3H]胸腺嘧啶核苷（TdR）和[125I] - 碘脱氧尿苷（IUdR）标记DNA的肠核蛋白制剂、脾细胞核、小鼠胸腺细胞或人肾T细胞。由于游离TdR比外源性DNA酶解产生的游离IUdR更有效地被再利用，受体小鼠组织DNA部分中[3H]TdR/[125I]IUdR的比值可衡量组织中完整外源性DNA的量。在大多数情况下，双标记的外源性DNA在注射后1天内几乎完全水解，但在某些情况下可证明全细胞DNA的存活。

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本文引用的文献

1
DEOXYRIBONUCLEIC ACID METABOLISM IN VIVO: I. CELL PROLIFERATION AND DEATH AS MEASURED BY INCORPORATION AND ELIMINATION OF IODODEOXYURIDINE.体内脱氧核糖核酸代谢：I. 通过碘脱氧尿苷的掺入和消除来测定细胞增殖与死亡
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THE FATE OF DNA-CONTAINING PARTICLES PHAGOCYTIZED BY MAMMALIAN CELLS.被哺乳动物细胞吞噬的含DNA颗粒的命运
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UPTAKE OF MAMMALIAN CHROMOSOMES BY MAMMALIAN CELLS.哺乳动物细胞对哺乳动物染色体的摄取
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Template properties of liver chromatin.肝脏染色质的模板特性
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A radioautographic study of the utilization of deoxycytidine for the formation of deoxyribonucleic acid-thymine in lymphocytes.关于淋巴细胞中利用脱氧胞苷形成脱氧核糖核酸胸腺嘧啶的放射自显影研究。
J Cell Biol. 1970 Aug;46(2):403-5. doi: 10.1083/jcb.46.2.403.
6
Differences in reutilization of thymidine in hemopoietic and lymphopoietic tissues of the normal mouse.正常小鼠造血组织和淋巴细胞组织中胸苷再利用的差异。
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