用[3H]胸腺嘧啶核苷和[125I]碘脱氧尿苷进行双重标记作为一种在体内确定注射的DNA和细胞命运的方法。

Double labeling with [3H]thymidine and [125I]iododeoxyuridine as a method for determining the fate of injected DNA and cells in vivo.

作者信息

Myers D K, Feinendegen L E

出版信息

J Cell Biol. 1975 Nov;67(2PT.1):484-8. doi: 10.1083/jcb.67.2.484.

DOI:10.1083/jcb.67.2.484

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2109598/

Abstract

Mice were injected intravenously and intraperitoneally with preparations of intestinal nucleoprotein, spleen nuclei, mouse thymus cells, or human kidney T cells whose DNA had been labeled with both [3H]thymidine (TdR) and [125I]-iododeoxyuridine (IUdR). Since free TdR is reutilized more efficiently than free IUdR produced by enzymic hydrolysis of the exogenous DNA, the ratio of [3H]TdR/[125I]IUdR in the DNA fraction of the tissues of the recipient mice provides a measure of the amount of intact exogenous DNA in the tissue. In most instances, the doubly labeled exogenous DNA was almost completely hydrolyzed within 1 day injection, but survival of the DNA from whole cells could be demonstrated in some cases.

摘要

给小鼠静脉内和腹腔内注射经[3H]胸腺嘧啶核苷（TdR）和[125I] - 碘脱氧尿苷（IUdR）标记DNA的肠核蛋白制剂、脾细胞核、小鼠胸腺细胞或人肾T细胞。由于游离TdR比外源性DNA酶解产生的游离IUdR更有效地被再利用，受体小鼠组织DNA部分中[3H]TdR/[125I]IUdR的比值可衡量组织中完整外源性DNA的量。在大多数情况下，双标记的外源性DNA在注射后1天内几乎完全水解，但在某些情况下可证明全细胞DNA的存活。

相似文献

1

Double labeling with [3H]thymidine and [125I]iododeoxyuridine as a method for determining the fate of injected DNA and cells in vivo.用[3H]胸腺嘧啶核苷和[125I]碘脱氧尿苷进行双重标记作为一种在体内确定注射的DNA和细胞命运的方法。

J Cell Biol. 1975 Nov;67(2PT.1):484-8. doi: 10.1083/jcb.67.2.484.

2

Incorporation of thymidine and iododeoxyuridine into the DNA of mouse tissues.胸腺嘧啶核苷和碘脱氧尿苷掺入小鼠组织的DNA中。

Can J Physiol Pharmacol. 1975 Dec;53(6):1014-22. doi: 10.1139/y75-141.

3

DNA turnover and thymidine re-utilization in mouse tissues.小鼠组织中的DNA周转与胸苷再利用

Cell Tissue Kinet. 1976 May;9(3):215-21. doi: 10.1111/j.1365-2184.1976.tb01269.x.

4

Local re-utilization of thymidine in normal mouse tissues as measured with iododeoxyuridine.用碘脱氧尿苷测量正常小鼠组织中胸苷的局部再利用情况。

Cell Tissue Kinet. 1988 Nov;21(6):381-7. doi: 10.1111/j.1365-2184.1988.tb00797.x.

5

A comparative study on the uptake in vitro of [3H]thymidine, [131I]5-iodo-2'-deoxyuridine and [3H]deoxycytidine in mouse spleen cells using double isotope autoradiography.运用双同位素放射自显影术对小鼠脾细胞摄取[3H]胸腺嘧啶核苷、[131I]5-碘-2'-脱氧尿苷及[3H]脱氧胞苷的体外情况进行的比较研究。

J Immunol Methods. 1975;8(1-2):151-7. doi: 10.1016/0022-1759(75)90091-5.

6

Toxicity and mutagenicity of X-rays and [125I]dUrd or [3H]TdR incorporated in the DNA of human lymphoblast cells.X射线以及掺入人淋巴母细胞DNA中的[125I]脱氧尿苷或[3H]胸苷的毒性和致突变性。

Mutat Res. 1983 Nov;111(3):387-404. doi: 10.1016/0027-5107(83)90035-0.

7

Proliferation of subependymal cells in the adult primate CNS: differential uptake of DNA labelled precursors.成年灵长类中枢神经系统室管膜下细胞的增殖：DNA标记前体的差异摄取

J Hirnforsch. 1983;24(1):23-33.

8

Fate of thymocytes: studies with 125I-iododeoxyuridine and 3H-thymidine in mice.胸腺细胞的命运：用125I-碘脱氧尿苷和3H-胸腺嘧啶核苷对小鼠进行的研究

Cell Tissue Kinet. 1977 Jan;10(1):57-69.

9

Comparison of DNA renewal in germ-free and conventional mice using [125I]iododeoxyuridine and [3H]thymidine.使用[125I]碘脱氧尿苷和[3H]胸苷对无菌小鼠和普通小鼠的DNA更新进行比较。

Cell Tissue Kinet. 1980 May;13(3):227-37. doi: 10.1111/j.1365-2184.1980.tb00462.x.

10

Double labeling autoradiography. Cell kinetic studies with 3H- and 14C-thymidine.双重标记放射自显影术。用³H-和¹⁴C-胸腺嘧啶核苷进行细胞动力学研究。

J Histochem Cytochem. 1981 Jan;29(1A Suppl):109-16.

引用本文的文献

1

Induction of lethal bystander effects in human breast cancer cell cultures by DNA-incorporated Iodine-125 depends on phenotype.DNA 结合碘-125 诱导人乳腺癌细胞培养中的致死旁观者效应取决于表型。

Int J Radiat Biol. 2012 Dec;88(12):1028-38. doi: 10.3109/09553002.2012.683511. Epub 2012 May 16.

2

Non-invasive analysis of metabolic reactions in body tissues, the case of myocardial fatty acids.人体组织中代谢反应的非侵入性分析，以心肌脂肪酸为例。

Eur J Nucl Med. 1981;6(5):191-200. doi: 10.1007/BF00290563.

本文引用的文献

1

DEOXYRIBONUCLEIC ACID METABOLISM IN VIVO: I. CELL PROLIFERATION AND DEATH AS MEASURED BY INCORPORATION AND ELIMINATION OF IODODEOXYURIDINE.体内脱氧核糖核酸代谢：I. 通过碘脱氧尿苷的掺入和消除来测定细胞增殖与死亡

Fed Proc. 1964 May-Jun;23:640-8.

2

THE FATE OF DNA-CONTAINING PARTICLES PHAGOCYTIZED BY MAMMALIAN CELLS.被哺乳动物细胞吞噬的含DNA颗粒的命运

J Cell Biol. 1964 Apr;21(1):105-14. doi: 10.1083/jcb.21.1.105.

3

UPTAKE OF MAMMALIAN CHROMOSOMES BY MAMMALIAN CELLS.哺乳动物细胞对哺乳动物染色体的摄取

J Cell Biol. 1963 Oct;19(1):71-7. doi: 10.1083/jcb.19.1.71.

4

Template properties of liver chromatin.肝脏染色质的模板特性

J Mol Biol. 1966 Jan;15(1):160-74. doi: 10.1016/s0022-2836(66)80218-8.

5

A radioautographic study of the utilization of deoxycytidine for the formation of deoxyribonucleic acid-thymine in lymphocytes.关于淋巴细胞中利用脱氧胞苷形成脱氧核糖核酸胸腺嘧啶的放射自显影研究。

J Cell Biol. 1970 Aug;46(2):403-5. doi: 10.1083/jcb.46.2.403.

6

Differences in reutilization of thymidine in hemopoietic and lymphopoietic tissues of the normal mouse.正常小鼠造血组织和淋巴细胞组织中胸苷再利用的差异。

Cell Tissue Kinet. 1973 Nov;6(6):573-85. doi: 10.1111/j.1365-2184.1973.tb01648.x.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

文档翻译

学术文献翻译模型，支持多种主流文档格式。