Kumegawa M, Takuma T, Murayama F
In Vitro. 1976 Oct;12(10):718-28. doi: 10.1007/BF02797476.
A new technique for organ culture which uses plastic culture chambers and the advantages of the cellophane-sheet technique is described with the results of a study of cultivations of fetal mouse liver. Two chambers, each containing cells, were placed in gas permeable roller tubes and rotated at 0.1 rpm in a CO2-air gassed incubator. The fetal mouse liver cells developed electron microscopic features similar to those of the in vivo adult liver by 9 days of cultivation. The albumin content and tyrosine aminotransferase (TAT) activity were detected in the cultivated liver. TAT activity was further induced by prednisolone. These results indicate that potential of this culture method for the study of physiological and pathological processes.
本文描述了一种利用塑料培养室及玻璃纸技术优点的器官培养新技术，并给出了对胎鼠肝脏培养研究的结果。将两个装有细胞的培养室置于透气的滚瓶中，在含二氧化碳的空气培养箱中以0.1转/分钟的速度旋转。培养9天后，胎鼠肝细胞呈现出与成年肝脏在体内相似的电子显微镜特征。在培养的肝脏中检测到白蛋白含量和酪氨酸转氨酶（TAT）活性。泼尼松龙进一步诱导了TAT活性。这些结果表明这种培养方法在研究生理和病理过程方面具有潜力。
