用于多功能培养室的循环系统。I. 关于一个12室（体外）封闭循环系统的力学、技术及基本结果的介绍
The circumfusion system for multipurpose culture chambers. I. Introduction to the mechanics, techniques, and basic results of a 12-chamber (in vitro) closed circulatory system.
作者信息
Rose G G
出版信息
J Cell Biol. 1967 Jan;32(1):89-112. doi: 10.1083/jcb.32.1.89.
Abstract
A self-contained mechanical system for circulating nutrient fluid through 12 tissue culture chambers is described in detail. This system utilizes nonperforated cellophane membranes in the chambers which separate the circulating nutrient from the tissue culture environments. The nutrient, therefore, is dialyzed through the cellophane of each chamber; some cell products are retained in the microenvironment between the closely apposed cellophane and cover slip, whereas the other cell products move from chamber to chamber in the circulating nutrient. The resultant environmental conditions directed by the circumfusion systems are highly favorable for maintaining the differentiation of chick embryo tissues over protracted periods; a number of micrographs are shown.
摘要
详细描述了一种用于使营养液在12个组织培养室中循环的独立机械系统。该系统在培养室中使用无孔玻璃纸膜，将循环的营养液与组织培养环境分隔开。因此，营养液通过每个培养室的玻璃纸进行透析；一些细胞产物保留在紧密贴合的玻璃纸和盖玻片之间的微环境中，而其他细胞产物则随着循环的营养液在培养室之间移动。由灌流系统引导产生的环境条件非常有利于在较长时间内维持鸡胚组织的分化；文中展示了一些显微照片。
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本文引用的文献
1
A method for studying the effect of P32 on living adult human epidermal cells in a perfusion chamber.
Tex Rep Biol Med. 1951;9(4):739-48.
2
The morphological diversity of Gey's strain HeLa after ten years in tissue culture.
Tex Rep Biol Med. 1962;20:308-37.
3
An unusual behavior of strain HeLa cells in tissue culture.
J Cell Biol. 1962 Apr;13(1):153-7. doi: 10.1083/jcb.13.1.153.
4
The design and performance of a perfusion system for the culture of the lens. Part II.
Arch Ophthalmol. 1960 Apr;63:607-24. doi: 10.1001/archopht.1960.00950020609003.
5
The effects of certain stimulants and depresants on individual fibroblasts in a perfusion chamber.
Ann N Y Acad Sci. 1954 Nov 17;58(7):1303-10. doi: 10.1111/j.1749-6632.1954.tb45910.x.
6
PHASE-CONTRAST MICROSCOPY IN LIVING CELLS.
J R Microsc Soc. 1964 Jun;83:97-114. doi: 10.1111/j.1365-2818.1964.tb00517.x.
7
NUCLEAR FOLDS VERSUS INTRANUCLEAR INCLUSIONS IN TISSUE CULTURES.
J R Microsc Soc. 1964 Dec;83:377-90. doi: 10.1111/j.1365-2818.1964.tb00555.x.
8
TIME-LAPSE CINEMICROGRAPHY OF CELLS IN TISSUE CULTURE.
Bull Johns Hopkins Hosp. 1965 Jan;116:33-68.
9
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J Dent Res. 1964 Jul-Aug;43:580-605. doi: 10.1177/00220345640430041301.
10
PERFUSION SYSTEM FOR REPLICATE MAMMALIAN CELL CULTURED IN T-60 FLASKS.
J Natl Cancer Inst. 1963 Jul;31:109-23.
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