Kolbinger W, Beyer C, Föhr K, Reisert I, Pilgrim C
Abteilung Anatomie und Zellbiologie, Universität Ulm, FRG.
Neuroendocrinology. 1992 Aug;56(2):148-52. doi: 10.1159/000126222.
In order to analyze the feedback action of prolactin (PRL) on the hypothalamus on the cellular level, we used primary cultures of rat embryonic diencephalon to measure the calcium response of individual neurons to PRL by means of fast fluorescence photometry. The cultures were subsequently stained with antibodies against the neuronal marker MAP-2, glutamic acid decarboxylase (GAD) or tyrosine hydroxylase (TH). PRL caused a rapid rise of intracellular free Ca2+ in a specific type of GABAergic neuron characterized by a spindle-shaped bipolar morphology and immunoreactivity to MAP-2 and GAD but not to TH. It is concluded that a subpopulation of hypothalamic GABAergic but not dopaminergic neurons react to PRL with a rapid increase in intracellular free Ca2+. These data are compatible with the assumption of a rapid negative feedback regulation of the secretion of PRL from the pituitary mediated by tuberoinfundibular GABAergic neurons.
为了在细胞水平分析催乳素（PRL）对下丘脑的反馈作用，我们使用大鼠胚胎间脑原代培养物，通过快速荧光光度法测量单个神经元对PRL的钙反应。随后，用针对神经元标志物微管相关蛋白2（MAP-2）、谷氨酸脱羧酶（GAD）或酪氨酸羟化酶（TH）的抗体对培养物进行染色。PRL导致一种特定类型的γ-氨基丁酸（GABA）能神经元内细胞内游离Ca2+迅速升高，这种神经元的特征是纺锤形双极形态，对MAP-2和GAD有免疫反应，但对TH没有免疫反应。得出的结论是，下丘脑GABA能神经元而非多巴胺能神经元的一个亚群对PRL作出反应，细胞内游离Ca2+迅速增加。这些数据与由结节漏斗GABA能神经元介导的垂体PRL分泌快速负反馈调节的假设相符。
