Gelot M A, Paysant P, Nabet P
C R Seances Soc Biol Fil. 1976;170(6):1248-55.
The authors have shown the presence in the human placenta of a binding activity for cAMP. This activity is protein like substances bound and is principaly located in cytosol and in nucleus. The authors have tried to purified this protein by gel filtration and chromatography on DEAE cellulose. Whatever the method employed, there is always in the protein a mixed activity: cAMP binding activity and HCS immunological activity. Purified HCS by classical method shows no binding activity. However when cAMP is added in immunological system HCS-immun-serum anti-HCS, the reaction is modified in an inhibitory way. This behaviour seem to be specific since never HGH nor TSH have the same. This behaviour has been confirmed several times as well during competition curves which shift toward left in presence of cAMP, as for assays of individual serums or amniotic fluids. In this last cases, the results are twice as eleveted depending if the lecture is done on a curve done without cAMP or with cAMP.
作者已证明人胎盘中存在对环磷酸腺苷（cAMP）的结合活性。这种活性与蛋白质样物质结合，主要位于细胞质和细胞核中。作者试图通过凝胶过滤和DEAE纤维素柱色谱法纯化这种蛋白质。无论采用何种方法，该蛋白质中总是存在混合活性：cAMP结合活性和人绒毛膜生长催乳素（HCS）免疫活性。用经典方法纯化的HCS无结合活性。然而，当在HCS免疫血清抗HCS的免疫系统中加入cAMP时，反应会以抑制方式改变。这种行为似乎具有特异性，因为生长激素（HGH）和促甲状腺激素（TSH）都没有相同的情况。在竞争曲线中，当存在cAMP时向左移动，以及在个体血清或羊水检测中，这种行为已被多次证实。在最后一种情况下，根据是在无cAMP还是有cAMP的曲线上进行读数，结果会高出两倍。
