SANCHEZ-MARROQUIN A
Appl Microbiol. 1963 Nov;11(6):523-8. doi: 10.1128/am.11.6.523-528.1963.
Gibberellic acid production from various substrates was studied in 43 strains of Fusarium, among which F. moniliforme strain IOC-3326 was selected as the best producer. Experiments were carried out in shaker flasks and pilot plant fermentors. The results indicate that the best substrate for gibberellic acid production with this strain is composed of the following: glucose, 20 g; corn steep liquor, 25 g; ammonium nitrate, 2.6 g; monopotassium phosphate, 0.5 g; potassium sulfate, 0.2 g; and water, 1000 ml. Glucose, ammonium nitrate, and corn steep liquor were found to be critical. With this medium, maximal yields of 1196 mg per liter in shaker flasks and 997 mg per liter in fermentors were produced.
对43株镰刀菌进行了研究,以考察它们利用各种底物生产赤霉素的情况,其中串珠镰刀菌IOC - 3326菌株被选为最佳生产菌株。实验在摇瓶和中试发酵罐中进行。结果表明,该菌株生产赤霉素的最佳底物组成如下:葡萄糖20克、玉米浆25克、硝酸铵2.6克、磷酸二氢钾0.5克、硫酸钾0.2克和水1000毫升。发现葡萄糖、硝酸铵和玉米浆是关键因素。使用这种培养基,摇瓶中每升的最大产量为1196毫克,发酵罐中每升为997毫克。