组织培养基的环氧乙烷灭菌
ETHYLENE OXIDE STERILIZATION OF TISSUE CULTURE MEDIA.
作者信息
BROWN B L, FUERST R
出版信息
Science. 1963 Dec 27;142(3600):1654-5. doi: 10.1126/science.142.3600.1654.
Abstract
Liquid ethylene oxide, which kills bacteria, yeasts, and molds, was added to a tissue culture medium instead of the usual antibiotics. With some limitations, the use of ethylene oxide sterilization of tissue culture medium has been shown feasible. Data indicate that this method can be used in experiments involving the sterilization of test chemicals and components of the medium used for mammalian cells in culture.
摘要
液态环氧乙烷能杀死细菌、酵母和霉菌，它被添加到组织培养基中以替代常用的抗生素。尽管存在一些局限性，但环氧乙烷对组织培养基进行灭菌的方法已被证明是可行的。数据表明，该方法可用于涉及对用于培养哺乳动物细胞的测试化学品和培养基成分进行灭菌的实验。
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