ENDERS A C, ENDERS R K, SCHLAFKE S
J Cell Biol. 1963 Aug;18(2):405-18. doi: 10.1083/jcb.18.2.405.
Portions of mink endometrium in delayed implantation, early postimplantation, and pseudo pregnancy were fixed in buffered osmium tetroxide with sucrose, or potassium permanganate. After rapid dehydration the portions of endometrium were embedded in either methacrylate or epoxy resin. Examination of the cells from the body of the glands of the endometrium of delayed implantation revealed the presence of prominent terminal bars, numerous secretion granules, and membrane discs in the apical region of the cell. In the supranuclear and infranuclear regions, mildly dilated cisternae of the endoplasmic reticulum were present, and in many cells unusually large mitochondria were seen. Numerous changes were noted in the gland cells of the post implantation stage. The endoplasmic reticulum in the basal region was extensively dilated, and the nuclei were situated more centrally. Giant mitochondria were no longer present. The large secretion granules were not present, but smaller granules were seen, especially in the Golgi region. Some of the Golgi cisternae were dilated and the pattern of parallel membranes was consequently less distinct. It is suggested that gland cells in the postimplantation and pseudopregnancy stages exhibit evidence of greater secretory activity than those in the delayed implantation stage.
将处于延迟着床、着床后早期和假孕状态的水貂子宫内膜部分用含蔗糖的缓冲四氧化锇或高锰酸钾固定。快速脱水后，将子宫内膜部分包埋在甲基丙烯酸酯或环氧树脂中。对延迟着床的子宫内膜腺体主体细胞的检查显示，细胞顶端区域存在明显的终末棒、大量分泌颗粒和膜盘。在核上和核下区域，内质网轻度扩张，许多细胞中可见异常大的线粒体。着床后阶段的腺细胞有许多变化。基部区域的内质网广泛扩张，细胞核位于更中央的位置。不再有巨大线粒体。大的分泌颗粒不存在，但可见较小的颗粒，尤其是在高尔基体区域。一些高尔基体池扩张，平行膜的模式因此不太明显。有人提出，着床后和假孕阶段的腺细胞比延迟着床阶段的腺细胞表现出更强的分泌活性迹象。
