EISENHARDT R H, ROSENTHAL O
Science. 1964 Jan 31;143(3605):476-7. doi: 10.1126/science.143.3605.476.
If the uncoupling agent 2,4-dinitrophenol is added just prior to addition of the phosphate acceptor adenosine diphosphate to rat liver mitochondria previously incubated with substrate, orthophosphate, and oxygen, steady-state phosphorylation is abolished, whereas the initial phase of rapid phosphorylation, the so-called "adenosine triphosphate jump," is undisturbed. This leads to the conclusion that uncoupling agents operate by interfering with the synthesis of high-energy intermediates, not by hydrolyzing or otherwise inactivating them.
如果在向预先用底物、正磷酸盐和氧气孵育过的大鼠肝线粒体中添加磷酸受体二磷酸腺苷之前，立即加入解偶联剂2,4-二硝基苯酚，稳态磷酸化就会被消除，而快速磷酸化的初始阶段，即所谓的“三磷酸腺苷跳跃”，则不受影响。由此得出结论，解偶联剂的作用是干扰高能中间体的合成，而不是通过水解或以其他方式使其失活。
