与lox重组位点结合的Cre重组酶的化学计量学。

Mack A, Sauer B, Abremski K, Hoess R

DuPont Merck Pharmaceutical Company, Experimental Station, Wilmington, DE 19880-0328.

Nucleic Acids Res. 1992 Sep 11;20(17):4451-5. doi: 10.1093/nar/20.17.4451.

The site-specific recombinase Cre from bacteriophage P1 binds and carries out recombination at a 34 bp lox site. The lox site consists of two 13 bp inverted repeats, separated by an 8 bp spacer region. Both the palindromic nature of the site and the results of footprinting and band shift experiments suggest that a minimum of two Cre molecules bind to a lox site. We report here experiments that demonstrate the absolute stoichiometry of the Cre-lox complex to be one molecule of Cre bound per inverted repeat, or two molecules per lox site.

来自噬菌体P1的位点特异性重组酶Cre在34 bp的lox位点结合并进行重组。lox位点由两个13 bp的反向重复序列组成，中间间隔一个8 bp的间隔区。该位点的回文性质以及足迹实验和凝胶迁移实验的结果表明，至少两个Cre分子与一个lox位点结合。我们在此报告的实验证明，Cre-lox复合物的绝对化学计量比为每个反向重复序列结合一个Cre分子，即每个lox位点结合两个分子。