Tazi-Saad K, Chariot J, Rozé C
INSERM U239, Faculté de Médecine X. Bichat, Paris, France.
Peptides. 1992 Mar-Apr;13(2):233-9. doi: 10.1016/0196-9781(92)90102-9.
Previous studies of the control of pepsin secretion by neurohumoral agents showed some discrepancies between in vitro (isolated cells) and in vivo experiments. In the present work, the effects on pepsin secretion of CCK, pentagastrin, secretin, VIP, neurotensin, histamine, and methacholine were reinvestigated in conscious gastric fistula rats, in comparison to acid secretion. ED50's and doses inducing maximal responses were measured to directly compare the potency and efficacy of these substances. Methacholine was the most efficient (maximal response = 4.5 x basal level, ED50 = 1.3 mumol/kg.h) and CCK the most potent (ED50 = 1.9 nmol/kg.h) stimulant, whereas secretin was a potent (ED50 regulators of pepsin secretion in the rat. Pentagastrin and histamine did not stimulate pepsin output, as found by others with isolated chief cells in vitro. Neurotensin and large doses of VIP marginally inhibited pepsin secretion.
以往关于神经体液因子对胃蛋白酶分泌控制的研究表明，体外（分离细胞）实验和体内实验之间存在一些差异。在本研究中，与胃酸分泌相比，在清醒胃瘘大鼠中重新研究了胆囊收缩素（CCK）、五肽胃泌素、促胰液素、血管活性肠肽（VIP）、神经降压素、组胺和乙酰甲胆碱对胃蛋白酶分泌的影响。测量了半数有效剂量（ED50）和诱导最大反应的剂量，以直接比较这些物质的效力和效能。乙酰甲胆碱是最有效的刺激物（最大反应 = 基础水平的4.5倍，ED50 = 1.3 μmol/kg·h），CCK是最有效的刺激物（ED50 = 1.9 nmol/kg·h），而促胰液素是大鼠胃蛋白酶分泌的有效调节剂（ED50……）。如其他人在体外分离的主细胞实验中所发现的那样，五肽胃泌素和组胺不刺激胃蛋白酶分泌。神经降压素和大剂量的VIP对胃蛋白酶分泌有轻微抑制作用。
