Colecchia M, Leopardi O
Department of Pathology, Ospedale Maggiore Lodi, Italy.
Pathol Res Pract. 1992 Jun;188(4-5):541-4. doi: 10.1016/S0344-0338(11)80052-X.
The authors have evaluated in eight reactive and eight malignant pleural effusions the number of intranuclear dots representing the nucleolar associated proteins stained with silver colloid technique (interphase nucleolar organizer regions-AgNORs). The mean number per nucleus in benign reactive effusions was 1.56 (SD 0.77) while in mesotheliomatous effusions it was 2.81 (SD 1.44). The statistical analysis of values, by Mann-Whitney U Wilcoxon Rank Sum W Test, revealed a significant difference of AgNOR counts in the two cytological samples. The variability of AgNOR areas and morphologies in reactive and mesotheliomatous nuclei in pleural fluids is evaluated applying automatic image analysis.
作者对8例反应性胸腔积液和8例恶性胸腔积液进行了评估，采用银胶体技术（间期核仁组织区-银染核仁组成区[AgNORs]）对代表核仁相关蛋白的核内点数量进行计数。良性反应性胸腔积液中每个细胞核的平均数量为1.56（标准差0.77），而在间皮瘤性胸腔积液中为2.81（标准差1.44）。通过曼-惠特尼U检验（威尔科克森秩和检验）对数值进行统计分析，结果显示这两种细胞学样本中的AgNOR计数存在显著差异。应用自动图像分析评估胸腔积液中反应性和间皮瘤性细胞核中AgNOR面积和形态的变异性。
