Rodenacker K, Aubele M, Burger G, Gais P, Jütting U, Gössner W, Oberholzer M
Institut für Strahlenschutz, Forschungszentrum für Umwelt und Gesundheit, Neuherberg, FRG.
Pathol Res Pract. 1992 Jun;188(4-5):556-60. doi: 10.1016/S0344-0338(11)80055-5.
A short description of a project of cytometry in histological sections of colon carcinoma is given with emphasis on the methodical aspects. Possible strategies of cytometric measurement and problems related to it (focus, overlap, segmentation of objects) are described. The main effort concerns interactive selection of tumor cells and the segmentation in cases of densely distributed and overlapping nuclei. All other succeeding processing steps are performed fully automatically. The resulting quantitative features are stored together with the original images on an optical disk for further examinations and reexaminations, allowing the direct relation of feature values to visual image content. The evaluation of the features as well as their interpretation is only at the beginning. Especially the problem of relating section information with true 3-dimensional information is not described here and necessitates further research. In a first investigation only a few tumors without and with metastases were analyzed. The preliminary results correspond with findings of Kunze et al.
本文简要介绍了一项关于结肠癌组织切片细胞计数的项目，重点阐述了方法学方面的内容。描述了细胞计数测量的可能策略及其相关问题（聚焦、重叠、物体分割）。主要工作涉及肿瘤细胞的交互式选择以及细胞核密集分布和重叠情况下的分割。所有后续处理步骤均完全自动执行。所得定量特征与原始图像一起存储在光盘上，以便进一步检查和复查，从而使特征值与视觉图像内容直接相关。对这些特征的评估及其解释尚处于起步阶段。特别是将切片信息与真实三维信息相关联的问题在此未作描述，需要进一步研究。在首次调查中，仅分析了少数有无转移的肿瘤。初步结果与Kunze等人的研究结果相符。
