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通过Mlu I细胞周期框对酵母DNA复制基因的调控依赖于SWI6。

Regulation of the yeast DNA replication genes through the Mlu I cell cycle box is dependent on SWI6.

作者信息

Verma R, Smiley J, Andrews B, Campbell J L

机构信息

Braun Laboratories, California Institute of Technology, Pasadena 91125.

出版信息

Proc Natl Acad Sci U S A. 1992 Oct 15;89(20):9479-83. doi: 10.1073/pnas.89.20.9479.

DOI:10.1073/pnas.89.20.9479
PMID:1409658
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC50155/
Abstract

In Saccharomyces cerevisiae, at least 17 DNA replication genes are coordinately expressed at the G1/S boundary during the cell cycle. All of these genes have the DNA sequence element ACGCGT in their 5' upstream regulatory regions. This sequence has been shown to be essential for periodic expression of the POL1, CDC9, and TMP1 genes. The cyclin (CLN1 and CLN2) and HO genes are another subset of genes that are expressed with the same timing as the DNA replication genes. Their periodic expression requires the participation of two well-characterized transcriptional activators: the SWI4 and SWI6 gene products. In this study, we present evidence that SWI6 contributes to the regulation of DNA replication genes as well. Surprisingly, a preferential requirement for SWI6 over SWI4 is observed in our studies of ACGCGT-dependent reporter gene expression in vivo. This selectivity has not been observed for the other G1/S genes. Correlating with the in vivo results, protein-DNA complexes formed in vitro on multimeric ACGCGT elements are either abolished or reduced in swi6 delta deletion mutants.

摘要

在酿酒酵母中，至少有17个DNA复制基因在细胞周期的G1/S边界处协调表达。所有这些基因在其5'上游调控区域都有DNA序列元件ACGCGT。已证明该序列对于POL1、CDC9和TMP1基因的周期性表达至关重要。细胞周期蛋白（CLN1和CLN2）和HO基因是与DNA复制基因同时表达的另一组基因。它们的周期性表达需要两种特性明确的转录激活因子的参与：SWI4和SWI6基因产物。在本研究中，我们提供证据表明SWI6也有助于DNA复制基因的调控。令人惊讶的是，在我们对体内ACGCGT依赖性报告基因表达的研究中，观察到对SWI6的需求优先于SWI4。对于其他G1/S基因，尚未观察到这种选择性。与体内结果相关的是，在体外多聚体ACGCGT元件上形成的蛋白质-DNA复合物在swi6δ缺失突变体中要么被消除，要么减少。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/111d/50155/2a1a2755cde6/pnas01094-0126-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/111d/50155/5e680f3a4456/pnas01094-0125-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/111d/50155/035581368b2c/pnas01094-0125-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/111d/50155/2dd93b93f6c3/pnas01094-0125-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/111d/50155/96a9c62beb93/pnas01094-0126-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/111d/50155/2a1a2755cde6/pnas01094-0126-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/111d/50155/5e680f3a4456/pnas01094-0125-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/111d/50155/035581368b2c/pnas01094-0125-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/111d/50155/2dd93b93f6c3/pnas01094-0125-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/111d/50155/96a9c62beb93/pnas01094-0126-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/111d/50155/2a1a2755cde6/pnas01094-0126-b.jpg

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1
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2
Transformation of intact yeast cells treated with alkali cations.经碱金属阳离子处理的完整酵母细胞的转化
J Bacteriol. 1983 Jan;153(1):163-8. doi: 10.1128/jb.153.1.163-168.1983.
3
Isolation of the gene encoding yeast DNA polymerase I.酵母DNA聚合酶I编码基因的分离
检查点激酶调节 DNA 损伤反应中的转录因子的全局网络。
Cell Rep. 2013 Jul 11;4(1):174-88. doi: 10.1016/j.celrep.2013.05.041. Epub 2013 Jun 27.
4
Pph3 dephosphorylation of Rad53 is required for cell recovery from MMS-induced DNA damage in Candida albicans.在白色念珠菌中，Pph3 对 Rad53 的去磷酸化作用是细胞从 MMS 诱导的 DNA 损伤中恢复所必需的。
PLoS One. 2012;7(5):e37246. doi: 10.1371/journal.pone.0037246. Epub 2012 May 14.
5
G1/S transcription factor orthologues Swi4p and Swi6p are important but not essential for cell proliferation and influence hyphal development in the fungal pathogen Candida albicans.G1/S转录因子同源物Swi4p和Swi6p对细胞增殖很重要，但并非必不可少，且会影响真菌病原体白色念珠菌的菌丝发育。
Eukaryot Cell. 2011 Mar;10(3):384-97. doi: 10.1128/EC.00278-10. Epub 2011 Jan 21.
6
New mutant versions of yeast FACT subunit Spt16 affect cell integrity.酵母 FACT 亚基 Spt16 的新型突变体版本影响细胞完整性。
Mol Genet Genomics. 2009 Nov;282(5):487-502. doi: 10.1007/s00438-009-0480-4. Epub 2009 Sep 1.
7
The Schizosaccharomyces pombe cdt2(+) gene, a target of G1-S phase-specific transcription factor complex DSC1, is required for mitotic and premeiotic DNA replication.粟酒裂殖酵母cdt2(+)基因是G1-S期特异性转录因子复合物DSC1的一个靶标，是有丝分裂和减数分裂前DNA复制所必需的。
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Mol Cell Biol. 2003 Jul;23(14):5064-77. doi: 10.1128/MCB.23.14.5064-5077.2003.
9
Complex transcriptional circuitry at the G1/S transition in Saccharomyces cerevisiae.酿酒酵母中G1/S转换处的复杂转录调控网络。
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4
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5
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10
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