Lambert G M, Rubin B S, Baum M J
Department of Biology, Boston University, MA 02215.
Physiol Behav. 1992 Oct;52(4):809-13. doi: 10.1016/0031-9384(92)90418-2.
A sexually dimorphic pattern in the secretion of luteinizing hormone (LH) has previously been shown to occur in response to mating in an induced ovulating species, the ferret, with mating augmenting the secretion of LH in females but not in males. The aim of this study was to determine whether this dimorphic pattern in the postcoital secretion of LH reflects a dimorphic effect of mating on the neural release of luteinizing hormone releasing hormone (LHRH). The effect of mating on the in vitro release of LHRH from mediobasal hypothalami (MBH) collected from breeding male and female ferrets was studied. Luteinizing hormone releasing hormone release and content were significantly reduced in tissues from estrous females sacrificed 0.25 h after mating compared to unpaired estrous females and estrous females sacrificed 1 or 2.6 h after the mating stimulus. By contrast, the release of LHRH from MBH fragments and LHRH tissue content were equivalent in breeding males that were sacrificed 0.25 h after mating and in breeding males that were left unpaired. These data suggest that the postcoital surge of LH in the female ferret is preceded by a release of LHRH that initially depletes neuronal terminals within the MBH, whereas LHRH release, like pituitary LH secretion, is minimally affected by mating in males.
先前的研究表明，在诱导排卵的雪貂物种中，促黄体生成素（LH）的分泌存在性别二态性模式，交配会增加雌性雪貂LH的分泌，但对雄性雪貂没有影响。本研究的目的是确定LH交配后分泌中的这种二态性模式是否反映了交配对促性腺激素释放激素（LHRH）神经释放的二态性影响。研究了交配对从繁殖期雄性和雌性雪貂收集的中基底下丘脑（MBH）中LHRH体外释放的影响。与未配对的发情雌性雪貂以及在交配刺激后1小时或2.6小时处死的发情雌性雪貂相比，在交配后0.25小时处死的发情雌性雪貂组织中，促性腺激素释放激素的释放和含量显著降低。相比之下，在交配后0.25小时处死的繁殖期雄性雪貂和未配对的繁殖期雄性雪貂中，MBH碎片中LHRH的释放和LHRH组织含量相当。这些数据表明，雌性雪貂交配后LH的激增之前有LHRH的释放，LHRH最初会耗尽MBH内的神经元终末，而LHRH的释放与垂体LH分泌一样，受雄性交配的影响最小。
