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枯草芽孢杆菌孢子形成过程中几种脱氢酶和转氨酶的放射敏感性

RADIOSENSITIVITY OF SEVERAL DEHYDROGENASES AND TRANSAMINASES DURING SPOROGENESIS OF BACILLUS SUBTILIS.

作者信息

ROWLEY D B, NEWCOMB H R

出版信息

J Bacteriol. 1964 Mar;87(3):701-9. doi: 10.1128/jb.87.3.701-709.1964.

Abstract

Rowley, D. B. (Syracuse University, Syracuse, N.Y.), and H. R. Newcomb. Radiosensitivity of several dehydrogenases and transaminases during sporogenesis of Bacillus subtilis. J. Bacteriol. 87:701-709. 1964.-Cells of Bacillus subtilis grown in a chemically defined medium for 17 hr, subsequently washed, and transferred to a phosphate solution containing CaCl(2) sporulated in a reproducible pattern. The synthesis of dipicolinic acid essentially paralleled the appearance of thermoresistant cells. X-ray resistant cells appeared 2 to 3 hr before the appearance of thermoresistant cells and the formation of dipicolinic acid. Changes in enzyme activity of cells undergoing sporogenesis varied among the enzymes studied. The transaminases exhibited a marked decrease in activity during early sporogenesis, whereas the dehydrogenases exhibited maximal activity. The determination of enzyme radioresistance in extracts of sporulating cells indicated that the resistance of transaminases remained essentially unchanged, whereas the dehydrogenases exhibited a decreased resistance early in sporogenesis. Although both glutamic-pyruvic transaminase and glutamic-oxaloacetic transaminase (GOT) were demonstrated in vegetative cells, only the latter was detected in mature spores. The radioresistance of GOT in vegetative-cell extracts was two to three times greater than in spore extracts.

摘要

罗利,D. B.(纽约州锡拉丘兹市雪城大学)和H. R. 纽科姆。枯草芽孢杆菌孢子形成过程中几种脱氢酶和转氨酶的放射敏感性。《细菌学杂志》87:701 - 709。1964年。——在化学成分确定的培养基中培养17小时的枯草芽孢杆菌细胞,随后洗涤并转移至含有氯化钙的磷酸盐溶液中,以可重复的模式形成孢子。吡啶二羧酸的合成基本上与耐热细胞的出现同步。抗X射线细胞在耐热细胞和吡啶二羧酸形成之前2至3小时出现。在孢子形成过程中,所研究的细胞的酶活性变化因酶而异。转氨酶在孢子形成早期活性显著下降,而脱氢酶则表现出最大活性。对正在形成孢子的细胞提取物中酶的抗辐射性测定表明,转氨酶的抗性基本保持不变,而脱氢酶在孢子形成早期抗性降低。虽然在营养细胞中同时检测到谷丙转氨酶和谷草转氨酶(GOT),但在成熟孢子中仅检测到后者。营养细胞提取物中GOT的抗辐射性比孢子提取物中的大两到三倍。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9b0/277074/ed862138512d/jbacter00443-0238-a.jpg

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