NUSSENZWEIG V, BENACERRAF B
J Exp Med. 1964 Mar 1;119(3):409-23. doi: 10.1084/jem.119.3.409.
The electrophoretic mobilities of guinea pig gamma(1) and gamma(2) antibodies bearing different specificities were compared in agar gel at pH 8.2. Specifically purified antibodies bearing the same immunological specificity showed the same electrophoretic mobility, but significant differences in mobility were observed when antibodies with certain selected different specificities were compared. The specificity of the carrier protein appeared not to affect the mobilities of antihapten antibodies. Differences in mobility have also been shown between gamma(1) antihapten antibodies produced by individual guinea pigs immunized concomitantly with 2,4-dinitrophenyl bovine gamma globulin and arsanilic acid azo guinea pig albumin. The differences in the net electrophoretic charge between antibodies with different specificities roughly paralleled that of their S fragments produced by papain digestion.
在pH 8.2的琼脂凝胶中比较了具有不同特异性的豚鼠γ(1)和γ(2)抗体的电泳迁移率。具有相同免疫特异性的经过特定纯化的抗体显示出相同的电泳迁移率,但当比较某些选定的具有不同特异性的抗体时,观察到迁移率存在显著差异。载体蛋白的特异性似乎不影响抗半抗原抗体的迁移率。在用2,4-二硝基苯基牛γ球蛋白和对氨基苯胂酸偶氮豚鼠白蛋白同时免疫的个体豚鼠产生的γ(1)抗半抗原抗体之间也显示出迁移率的差异。具有不同特异性的抗体之间净电泳电荷的差异大致与其经木瓜蛋白酶消化产生的S片段的差异平行。