Elghetany M T, Sullivan A K, Kurec A S, MacCallum J M, Bloomfield C D, Sobol R E, Davey F R
Department of Pathology, SUNY Health Science Center, Syracuse 13210.
Am J Clin Pathol. 1992 Oct;98(4):430-6. doi: 10.1093/ajcp/98.4.430.
Three monoclonal antibodies, K101, D46, and H36/71 (CD15), reactive with membrane components of primary granules of human promyelocytes, were studied to assess their binding to normal and leukemic cells. Using the alkaline phosphatase antialkaline phosphatase technique, these antibodies were applied to sections of normal organs and to peripheral blood and bone marrow films from hematologically normal individuals and patients with hematologic malignancies. In control experiments, antibodies showed reactivity with cytoplasmic constituents of granulocytes from the promyelocytic to the neutrophilic stage. In acute myeloid leukemia, antibody K101 was positive (more than 20% of blasts) in 13 of 21 (62%) cases, while antibody D46 was positive in 11 of 17 (65%) cases. Antibody H36/71 was positive in only 4 of 24 (17%) cases of acute myeloid leukemia. At least one marker was present in 6 of 8 (75%) cases of acute lymphoblastic leukemia with myeloid antigen-positive blasts and was negative in 20 cases of acute lymphoblastic leukemia with myeloid antigen-negative blasts. These results support the view that abnormal granules (with defective expression of the D46, K101, and H36/71 antigens) form in blastic and leukemic cells of patients with acute myeloid leukemia. Data also suggest that membrane components of myeloid granules are made in the cytoplasm of cells from some acute lymphoblastic leukemia patients with myeloid antigen-positive blasts.
研究了三种与人类早幼粒细胞初级颗粒膜成分发生反应的单克隆抗体K101、D46和H36/71（CD15），以评估它们与正常细胞和白血病细胞的结合情况。采用碱性磷酸酶抗碱性磷酸酶技术，将这些抗体应用于正常器官切片以及血液学正常个体和血液系统恶性肿瘤患者的外周血和骨髓涂片。在对照实验中，抗体显示出与从早幼粒细胞到中性粒细胞阶段的粒细胞胞质成分发生反应。在急性髓系白血病中，抗体K101在21例中的13例（62%）呈阳性（超过20%的原始细胞），而抗体D46在17例中的11例（65%）呈阳性。抗体H36/71在24例急性髓系白血病中仅4例（17%）呈阳性。在8例具有髓系抗原阳性原始细胞的急性淋巴细胞白血病中，6例（75%）至少有一个标志物呈阳性，而在20例具有髓系抗原阴性原始细胞的急性淋巴细胞白血病中呈阴性。这些结果支持这样一种观点，即急性髓系白血病患者的原始细胞和白血病细胞中形成了异常颗粒（D46、K101和H36/71抗原表达缺陷）。数据还表明，一些具有髓系抗原阳性原始细胞的急性淋巴细胞白血病患者细胞的细胞质中产生了髓系颗粒的膜成分。
