Ball E D, McDermott J, Griffin J D, Davey F R, Davis R, Bloomfield C D
Department of Medicine, Dartmouth Medical School, Hanover, NH 03756.
Blood. 1989 May 15;73(7):1951-6.
Monoclonal antibodies (MoAbs) have been prepared recently that recognize the three cell-surface receptors for the Fc portion of immunoglobulin (Ig), termed Fc gamma RI (MoAb 32.2), Fc gamma R II (MoAb IV-3), and Fc gamma R III (MoAb 3G8) that are expressed on selected subsets of non-T lymphocyte peripheral blood leukocytes. In the blood, Fc gamma R I is expressed exclusively on monocytes and macrophages, Fc gamma R II on granulocytes, mononuclear phagocytes, platelets, and B cells, and Fc gamma R III on granulocytes and natural killer (NK) cells. We have examined the expression of these molecules on normal bone marrow (BM) cells and on leukemia cells from the blood and/or BM in order to determine their normal ontogeny as well as their distribution on leukemic cells. BM was obtained from six normal volunteers and from 170 patients with newly diagnosed acute leukemia. Normal BM cells were found to express Fc gamma R I, II, and III with the following percentages: 40%, 58%, and 56%, respectively. Cell sorting revealed that both Fc gamma R I and Fc gamma R II were detectable on all subclasses of myeloid precursors as early as myeloblasts. Cell sorting experiments revealed that 66% of the granulocyte-monocyte colony-forming cells (CFU-GM) and 50% of erythroid burst-forming units (BFU-E) were Fc gamma R II positive with only 20% and 28%, respectively, of CFU-GM and BFU-E were Fc gamma R I positive. Acute myeloid leukemia (AML) cells expressed the three receptors with the following frequency (n = 146): Fc gamma R I, 58%; Fc gamma R II, 67%; and Fc gamma R III, 26% of patients. Despite the fact that Fc gamma R I is only expressed on monocytes among blood cells, AML cells without monocytoid differentiation (French-American-British [FAB]M1, M2, M3, M6) were sometimes positive for this receptor. However, Fc gamma R I was highly correlated with FAB M4 and M5 morphology (P less than .001). Fc gamma R II was also correlated with FAB M4 and M5 morphology (P = .003). Cells from 11 patients with acute lymphoblastic leukemia were negative for Fc gamma R I, but six cases were positive for Fc gamma R II and III (not the same patients). These studies demonstrate that Ig Fc gamma R are acquired during normal differentiation in the BM at or before the level of colony-forming units. In addition, we show that acute leukemia cells commonly express Fc gamma R.
最近制备了单克隆抗体（MoAbs），它们可识别免疫球蛋白（Ig）Fc部分的三种细胞表面受体，即FcγRI（单克隆抗体32.2）、FcγR II（单克隆抗体IV - 3）和FcγR III（单克隆抗体3G8），这些受体在非T淋巴细胞外周血白细胞的特定亚群上表达。在血液中，FcγR I仅在单核细胞和巨噬细胞上表达，FcγR II在粒细胞、单核吞噬细胞、血小板和B细胞上表达，FcγR III在粒细胞和自然杀伤（NK）细胞上表达。我们检测了这些分子在正常骨髓（BM）细胞以及血液和/或BM来源的白血病细胞上的表达情况，以确定它们的正常个体发生以及在白血病细胞上的分布。从6名正常志愿者和170例新诊断的急性白血病患者获取了BM。发现正常BM细胞表达FcγR I、II和III，其百分比分别为：40%、58%和56%。细胞分选显示，早在成髓细胞阶段，FcγR I和FcγR II在髓系前体细胞的所有亚类上均可检测到。细胞分选实验显示，66%的粒细胞 - 单核细胞集落形成细胞（CFU - GM）和50%的红系爆式集落形成单位（BFU - E）为FcγR II阳性，而CFU - GM和BFU - E中分别只有20%和28%为FcγR I阳性。急性髓系白血病（AML）细胞表达这三种受体的频率如下（n = 146）：FcγR I，58%；FcγR II，67%；FcγR III，26%的患者。尽管FcγR I仅在血细胞中的单核细胞上表达，但无单核细胞样分化的AML细胞（法 - 美 - 英[FAB]M1、M2、M3、M6）有时该受体呈阳性。然而，FcγR I与FAB M4和M5形态高度相关（P <.001）。FcγR II也与FAB M4和M5形态相关（P =.003）。11例急性淋巴细胞白血病患者的细胞FcγR I为阴性，但6例FcγR II和III为阳性（并非同一患者）。这些研究表明，Ig FcγR在BM中正常分化过程中在集落形成单位水平或之前获得。此外，我们表明急性白血病细胞通常表达FcγR。
