KAPLAN M H, SUCHY M L
J Exp Med. 1964 Apr 1;119(4):643-50. doi: 10.1084/jem.119.4.643.
Further evidence of a cross-reactive relationship between a cell wall antigen of certain Group A streptococcal strains and mammalian heart tissue is provided by the demonstration that goat antisera to washed human or rabbit heart tissue homogenates exhibit precipitation with cell wall extract preparations. A single line of precipitation was observed in agar gel diffusion tests. Precipitating activity of goat antiheart sera was related to the gamma-globulin fraction in zone electrophoresis. The cell wall antigen was demonstrated in cell wall extracts of a Type 5 and Type 19 but not of a Type 12 strain. Antigenic activity was concentrated in the same neutral salt and chromatographic fractions of acid extracts of cell wall previously reported to exhibit this activity by immunofluorescent inhibition of the cross-reaction of antistreptococcal serum with heart tissue.
某些A组链球菌菌株的细胞壁抗原与哺乳动物心脏组织之间存在交叉反应关系的进一步证据是,通过证明山羊抗人或兔心脏组织匀浆的抗血清与细胞壁提取物制剂呈现沉淀反应来提供的。在琼脂凝胶扩散试验中观察到一条沉淀线。山羊抗心脏血清的沉淀活性与区带电泳中的γ-球蛋白部分有关。在5型和19型菌株的细胞壁提取物中证明了该细胞壁抗原,但在12型菌株中未证明。抗原活性集中在细胞壁酸提取物的相同中性盐和色谱级分中,先前通过免疫荧光抑制抗链球菌血清与心脏组织的交叉反应报道该级分具有这种活性。