PIERCE W A
J Bacteriol. 1964 Oct;88(4):912-21. doi: 10.1128/jb.88.4.912-921.1964.
Pierce, William A., Jr. (Tulane University, New Orleans, La.). Electrophoretic separation of constituents of partially purified M protein of Streptococcus pyogenes. J. Bacteriol. 88:912-921. 1964.-Partially purified M protein of a group A, type 12 strain of Streptococcus pyogenes was studied by use of chemical, electrophoretic, and immunological techniques. It was demonstrated in immunodiffusion tests that the antigen contains multiple precipitating components. The type-specific antigen was identified, and evidence was presented that, in some instances at least, the cross-reactions observed between this type 12 M protein and heterologous antisera in immunodiffusion tests involve contaminating antigens rather than the component which precipitates with adsorbed homologous-typing antiserum. In passive hemagglutination tests where M protein was adsorbed to tanned sheep erythrocytes, it was found that antisera suitably adsorbed to show good specificity in capillary precipitin tests nevertheless still contain cross-reactive antibodies which are detectable by this more sensitive technique. Electrophoresis on starch paste separates some of the components of partially purified M protein, so that a fraction can be obtained which has fewer precipitating antigens, as determined in immunodiffusion tests, and which is less cross-reactive in passive hemagglutination tests with heterologous unadsorbed antistreptococcal antisera.
小皮尔斯·威廉·A.（杜兰大学，路易斯安那州新奥尔良）。化脓性链球菌部分纯化的M蛋白成分的电泳分离。《细菌学杂志》88:912 - 921。1964年。——利用化学、电泳和免疫学技术研究了A组12型化脓性链球菌菌株的部分纯化M蛋白。免疫扩散试验表明该抗原含有多种沉淀成分。鉴定出了型特异性抗原，并且有证据表明，至少在某些情况下，在免疫扩散试验中观察到的这种12型M蛋白与异源抗血清之间的交叉反应涉及污染抗原，而非与吸附的同源分型抗血清沉淀的成分。在将M蛋白吸附到鞣酸处理的绵羊红细胞上的被动血凝试验中，发现经过适当吸附以在毛细管沉淀素试验中显示出良好特异性的抗血清，通过这种更灵敏的技术仍可检测到其中含有交叉反应性抗体。在淀粉糊上进行电泳可分离部分纯化M蛋白的一些成分，从而可获得一个组分，在免疫扩散试验中其沉淀抗原较少，并且在用异源未吸附抗链球菌抗血清进行的被动血凝试验中交叉反应性较低。
