SHALLA T A
J Cell Biol. 1964 May;21(2):253-64. doi: 10.1083/jcb.21.2.253.
Cells of tomato leaflets (Lycopersicum esculentum Mill.) were studied by phase and electron microscopy at various intervals after inoculation with a common strain of tobacco mosaic virus (TMV). Forty-eight hours after inoculation, prior to the development of assayable virus, individual TMV particles, and also particle aggregates, were observed in the ground cytoplasm of mesophyll cells. The most rapid synthesis of virus occurred between 80 and 300 hours after inoculation. Cytological changes during this time were characterized by an increased number of individual particles in the cytoplasm, growth of some aggregates, distortion and vacuolation of chloroplasts, and formation of filaments in the cytoplasm which were approximately four times the size of TMV. These filaments were interpreted as possible developmental forms of the TMV particle. Vacuoles in chloroplasts commonly contained virus particles. Evidence indicated that TMV was assembled in the ground cytoplasm and, in some cases, subsequently was enveloped by distorted chloroplasts.
对接种烟草花叶病毒（TMV）常见毒株后的不同时间间隔，利用相差显微镜和电子显微镜对番茄小叶（番茄）细胞进行了研究。接种后48小时，在可检测到病毒出现之前，在叶肉细胞的基质细胞质中观察到了单个TMV粒子以及粒子聚集体。病毒合成最迅速的阶段发生在接种后80至300小时之间。在此期间的细胞学变化表现为细胞质中单个粒子数量增加、一些聚集体生长、叶绿体变形和液泡化，以及在细胞质中形成大小约为TMV四倍的丝状结构。这些丝状结构被解释为TMV粒子可能的发育形式。叶绿体中的液泡通常含有病毒粒子。有证据表明，TMV在基质细胞质中组装，在某些情况下，随后被变形的叶绿体包裹。
