Terashima M, Ikeda K, Kawamura H, Maesawa C, Takiyama I, Yoshinari H, Koeda K, Sato N, Ishida K, Saito K
Dept. of Surgery 1, Iwate Medical University School of Medicine.
Gan To Kagaku Ryoho. 1992 Oct;19(12):1999-2004.
Potentiation of cytotoxic effects of 5-fluorouracil (5-FU) were investigated by simultaneous or sequential combination of l-leucovorin (LV) against human esophageal cancer cell line (TE-1, TE-2) and 23 human clinical cancer samples in vitro. LV enhanced the cytotoxic effects of 5-FU on human esophageal cancer cell line as a dose dependent manner, and increased the cytotoxic effect of 5-FU about 1.5-fold with 10 microM and about 2-fold with 100 microM. The incubation time did not affect the effects. The potentiation with LV was also demonstrated against human clinical cancer samples, and the cytotoxic effects of 5-FU increased 7.6% in esophageal cancer, 20.9% in gastric cancer and 25.5% in colorectal cancer. As a result, the potentiating effects of LV against 5-FU seemed to be limited on human esophageal cancer.
通过左旋亚叶酸（LV）与5-氟尿嘧啶（5-FU）同时或序贯联合使用，在体外对人食管癌细胞系（TE-1、TE-2）和23份人类临床癌症样本研究了5-FU细胞毒性作用的增强情况。LV以剂量依赖方式增强了5-FU对人食管癌细胞系的细胞毒性作用，在10微摩尔时使5-FU的细胞毒性作用增加约1.5倍，在100微摩尔时增加约2倍。孵育时间不影响该作用。LV对人类临床癌症样本也显示出增强作用，5-FU的细胞毒性作用在食管癌中增加了7.6%，在胃癌中增加了20.9%，在结直肠癌中增加了25.5%。结果，LV对5-FU的增强作用在人类食管癌上似乎有限。
