Zheludkov M M, Gubina E A, Lebedeva I K, D'iakov S I
Antibiot Khimioter. 1992 May;37(5):12-4.
Optimal conditions were developed for determination of antibiotic sensitivity in Brucella by using enzyme immunoassay directly in the primary cultures of the material tested. The Brucella concentration in the material tested should be not lower than 1.10(6) microbial cells/ml and the time of culture incubation be 24 hours at 37 degrees C. The obligatory condition is to use a liquid medium, i.e. the Albimi broth with 1% glucose. To inhibit the foreign microflora it is recommended to use polymyxin B and amphoglucamine in a concentration of 3 microgram/ml. The use of enzyme immunoassay was shown that it was possible to determine the antibiotic sensitivity of Brucella in practice.
通过直接在受试材料的原代培养物中使用酶免疫测定法，确定了布鲁氏菌抗生素敏感性的最佳条件。受试材料中的布鲁氏菌浓度应不低于1.10(6)个微生物细胞/毫升，培养孵育时间为37℃下24小时。必要条件是使用液体培养基，即含1%葡萄糖的阿尔比米肉汤。为抑制外来微生物群，建议使用浓度为3微克/毫升的多粘菌素B和两性霉素。酶免疫测定法的应用表明，在实际中可以确定布鲁氏菌的抗生素敏感性。
