Lass J H, Bourne W M, Musch D C, Sugar A, Gordon J F, Reinhart W J, Meyer R F, Patel D I, Bruner W E, Cano D B
Department of Ophthalmology, Case Western Reserve University, Ohio 44106.
Arch Ophthalmol. 1992 Oct;110(10):1404-8. doi: 10.1001/archopht.1992.01080220066022.
We compared Optisol and DexSol, two chondroitin-sulfate-based media for corneal storage at 4 degrees C, by transplanting 31 donor cornea pairs (one cornea stored in Optisol and its mate in DexSol for 20 to 134 hours) into 31 pairs of recipients (62 patients). All grafts were clear 1 year after transplantation except for one primary donor failure (Optisol group). Optisol-stored corneas were significantly thinner than DexSol-stored corneas after cardinal suture placement (0.64 mm vs 0.76 mm) and at the end of surgery (0.69 mm vs 0.78 mm); at all points afterward through 1 year the two groups did not differ. The activity of two lysosomal enzymes released into the media during storage, alpha-mannosidase and alpha-glucosidase, was lower in the Optisol group. Epithelial status and all endothelial morphometric parameters, except the figure coefficient at 1 year, did not differ between the two groups before surgery and 3, 6, and 12 months after surgery. Mean endothelial cell loss at 1 year was 15% for the Optisol group and 21% for the DexSol group (P = .22). Thus, Optisol-stored corneas were thinner during surgery than DexSol-stored corneas and there was less lysosomal enzyme activity in the Optisol medium after tissue storage. There were no significant differences in postoperative clinical or endothelial morphometric parameters, however.
我们通过将31对供体角膜（一对角膜在Optisol中储存，另一对在DexSol中储存20至134小时）移植到31对受体（62名患者）中，比较了Optisol和DexSol这两种基于硫酸软骨素的4℃角膜储存介质。除了一例原发性供体失败（Optisol组）外，所有移植在术后1年时植片均透明。在放置主要缝线后（0.64毫米对0.76毫米）以及手术结束时（0.69毫米对0.78毫米），Optisol储存的角膜明显比DexSol储存的角膜薄；在之后直至1年的所有时间点，两组没有差异。储存期间释放到培养基中的两种溶酶体酶，α-甘露糖苷酶和α-葡萄糖苷酶的活性，在Optisol组中较低。术前以及术后3、6和12个月时，两组的上皮状态和所有内皮形态学参数（除了1年时的图形系数）没有差异。Optisol组1年时的平均内皮细胞丢失率为15%，DexSol组为21%（P = 0.22）。因此，Optisol储存的角膜在手术期间比DexSol储存的角膜薄，并且组织储存后Optisol培养基中的溶酶体酶活性较低。然而，术后临床或内皮形态学参数没有显著差异。
