Jerusalinsky D, Ferreira M B, Walz R, Da Silva R C, Bianchin M, Ruschel A C, Zanatta M S, Medina J H, Izquierdo I
Departamento de Bioquimica, UFRGS (centro), Porto Alegre, RS, Brazil.
Behav Neural Biol. 1992 Jul;58(1):76-80. doi: 10.1016/0163-1047(92)90982-a.
The blockers of glutamate receptors, aminophosphonovaleric acid (AP5) (5.0 micrograms) and cyano-nitroquinoxaline-dione (CNQX) (0.5 microgram), were infused bilaterally into the amygdala, dorsal hippocampus, or entorhinal cortex of rats through indwelling cannulae 0, 90, 180, or 360 min after step-down inhibitory avoidance training. Animals were tested for retention 24 h after training. In the amygdala or hippocampus, AP5 was amnestic when given 0 min after training and CNQX was amnestic when given 0, 90, or 180 min after training. In the entorhinal cortex, AP5 was amnestic when given 90 or 180 min after training and CNQX had no effect. The results suggest that a phenomenon sensitive first to AP5 and then to CNQX in the amygdala and hippocampus, probably long-term potentiation (LTP), is crucial to post-training memory processing. LTP in these two structures could underlie their role in memory consolidation and could explain the late involvement of the entorhinal cortex in post-training memory processing.
在大鼠进行递减式抑制性回避训练后0、90、180或360分钟，通过留置套管将谷氨酸受体阻滞剂——氨基磷酸戊酸（AP5）（5.0微克）和氰基硝基喹喔啉二酮（CNQX）（0.5微克）双侧注入杏仁核、背侧海马体或内嗅皮质。训练24小时后对动物进行记忆保持测试。在杏仁核或海马体中，训练后0分钟给予AP5会导致遗忘，训练后0、90或180分钟给予CNQX会导致遗忘。在内嗅皮质中，训练后90或180分钟给予AP5会导致遗忘，而CNQX则无影响。结果表明，杏仁核和海马体中一种先对AP5敏感、后对CNQX敏感的现象，可能是长时程增强（LTP），对训练后的记忆处理至关重要。这两个结构中的LTP可能是它们在记忆巩固中发挥作用的基础，并且可以解释内嗅皮质在训练后记忆处理中的后期参与。
