关于由偶氮 - 促黄体生成激素释放激素 - 破伤风类毒素（azo-LHRH-TT）和偶氮 - 促黄体生成激素释放激素 - 牛血清白蛋白（azo-LHRH-BSA）偶联物所制备的抗促黄体生成激素释放激素抗体的免疫特异性和亲和力研究。
Immunospecificity and affinity studies on anti-luteinizing hormone releasing hormone antibodies developed by azo-LHRH-TT and azo-LHRH-BSA conjugates.
作者信息
Singh V
机构信息
Institute of Self-Organising Systems and Biophysics, North-Eastern Hill University, Meghalaya, India.
出版信息
Biochem Int. 1992 Aug;27(4):591-9.
Monoclonal and conventional antibodies against the 'self' decapeptide LHRH produced by azo-LHRH-TT and azo-LHRH-BSA conjugates were studied for immunoreactivity with native LHRH, azo-LHRH, LHRH (OH) and its fragments of sequence 1-10, 7-10, 4-10 and 4-6. The CoAb reacted 8-20% to azo-LHRH, 2-6% to LHRH-Lys, 1-3% LHRH-LYS-MDP and 0.5-1.68% to LHRH-Ala-Ala-Tuftsin but failed to react to LHRH free acid and its fragments. On the other hand MoAb reacted 4-6% to azo-LHRH and 10-18% to LHRH-Lys, 3-5% to LHRH-Ala-Ala-Tuftsin. These results indicate that the azotization drastically inhibited immunoreactivity but that the azo-LHRH-carrier conjugate preserved the immunogenic epitope responsible for the induction of an effective anti-LHRH antibody response.
研究了由偶氮-LHRH-TT和偶氮-LHRH-BSA偶联物产生的针对“自身”十肽LHRH的单克隆抗体和常规抗体与天然LHRH、偶氮-LHRH、LHRH（OH）及其1-10、7-10、4-10和4-6序列片段的免疫反应性。CoAb对偶氮-LHRH的反应为8-20%，对LHRH-Lys的反应为2-6%，对LHRH-LYS-MDP的反应为1-3%，对LHRH-Ala-Ala-Tuftsin的反应为0.5-1.68%，但对游离LHRH酸及其片段无反应。另一方面，MoAb对偶氮-LHRH的反应为4-6%，对LHRH-Lys的反应为10-18%，对LHRH-Ala-Ala-Tuftsin的反应为3-5%。这些结果表明，偶氮化极大地抑制了免疫反应性，但偶氮-LHRH-载体偶联物保留了负责诱导有效抗LHRH抗体反应的免疫原性表位。
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