McDanell R E, Beales D, Henderson L, Sethi J K
Department of Medicine, Rayne Institute, University College London, U.K.
Biochem Pharmacol. 1992 Oct 6;44(7):1303-6. doi: 10.1016/0006-2952(92)90530-v.
In the present study, we have examined the effect of dietary fat on paracetamol-induced liver injury in an in vitro rat liver slice model. Rats were fed, for 7-10 days, diets containing either butter or polyunsaturated vegetable margarine, two fat sources commonly consumed in the human diet. Liver slices were then exposed to paracetamol for 2 hr and further incubated for 4 hr without paracetamol. Cell damage in the slices was quantified at 6 hr by measuring leakage of lactate dehydrogenase, increase in water content and potassium loss. Covalent binding of radioactive paracetamol to liver and the membrane fatty acid composition of the liver were also measured. Liver slices from rats fed butter diets were significantly more sensitive to the toxic effects of paracetamol than those from margarine fed rats. The membrane lipid composition of the livers also reflected the differing fatty acid content of the two diets.
在本研究中，我们在体外大鼠肝切片模型中研究了膳食脂肪对乙酰氨基酚诱导的肝损伤的影响。给大鼠喂食含黄油或多不饱和植物人造黄油的饲料7至10天，这是人类饮食中常见的两种脂肪来源。然后将肝切片暴露于乙酰氨基酚2小时，之后在无乙酰氨基酚的情况下再孵育4小时。在6小时时通过测量乳酸脱氢酶泄漏、含水量增加和钾流失来量化切片中的细胞损伤。还测量了放射性乙酰氨基酚与肝脏的共价结合以及肝脏的膜脂肪酸组成。喂食黄油饲料的大鼠的肝切片对乙酰氨基酚的毒性作用比喂食人造黄油的大鼠的肝切片更敏感。肝脏的膜脂质组成也反映了两种饮食中不同的脂肪酸含量。
