Trim A R, Dawson J R, Dickerson P E, Sakai F, Watts J W
Z Naturforsch C Biosci. 1977 May-Jun;32(5-6):424-8. doi: 10.1515/znc-1977-5-618.
Cowpea chlorotic mottle virus RNA has been prepared in comparatively high yield (at least 50%) by a modified phenol extraction method. The preparation, which has high biological activity, has been resolved into four components by zonal centrifugation on a 15-40% (w/v) sucrose density gradient. The components obtained have been tested for biological activity against whole plants and plant protoplasts. Each of the two largest components RNA 1 and RNA 2 was by itself infective (50-90% of the specific infectivity of the whole genome) and produced virus-specific proteins (coat protein and P2) and RNAs ("RNA 3" and "RNA 4"). Contamination by small proportions (less than 10%) of neighbouring RNAs is presumed to be involved in this infectivity. The two smallest components were obtained in an almost pure form.
通过改良的苯酚提取法，已以相对较高的产量（至少50%）制备了豇豆褪绿斑驳病毒RNA。该制备物具有较高的生物活性，通过在15-40%（w/v）蔗糖密度梯度上进行区带离心，已被分离成四个组分。已对所获得的组分针对整株植物和植物原生质体的生物活性进行了测试。两个最大的组分RNA 1和RNA 2各自具有感染性（为全基因组特异性感染性的50-90%），并产生病毒特异性蛋白质（外壳蛋白和P2）和RNA（“RNA 3”和“RNA 4”）。据推测，这种感染性与少量（小于10%）相邻RNA的污染有关。两个最小的组分几乎是以纯形式获得的。
