Tandon N, Jayne D R, McGregor A M, Weetman A P
Department of Medicine, University of Sheffield Clinical Sciences Centre, Northern General Hospital, UK.
J Autoimmun. 1992 Oct;5(5):557-70. doi: 10.1016/0896-8411(92)90153-h.
Anti-microsomal antibody (AMA) activity was inhibited in 14 of 16 sera and in all 12 IgG preparations from patients with postpartum thyroiditis following incubation with F(ab')2 fragments from normal polyspecific immunoglobulin for therapeutic use (ivIg). Similar results were observed with sera from seven of seven patients with Graves' disease and five of six patients with autoimmune hypothyroidism. Results of these competitive binding assays and affinity chromatography of AMA IgG on Sepharose-bound F(ab'), fragments from ivIg indicated that AMA antibodies reacted with ivIg through idiotypic-anti-idiotypic interactions. Eight out of 10 IgG preparations from patients with autoimmune thyroid disease also showed inhibition of AMA activity when coincubated with autologous IgM at various IgG:IgM molar ratios. These observations suggest that ivIg can inhibit anti-microsomal antibodies through idiotype-anti-idiotype interactions and that such interactions occur with IgM anti-idiotype antibodies in vivo, providing evidence of a role for idiotypic network regulation in the control of thyroid autoimmunity.
16份血清中的14份以及产后甲状腺炎患者的所有12份IgG制剂在与治疗用正常多特异性免疫球蛋白（静脉注射免疫球蛋白，ivIg）的F(ab')2片段孵育后，抗微粒体抗体（AMA）活性受到抑制。格雷夫斯病患者的7份血清以及自身免疫性甲状腺功能减退症患者的6份血清中的5份也观察到了类似结果。这些竞争性结合试验以及AMA IgG在与ivIg的琼脂糖结合F(ab')片段上进行亲和层析的结果表明，AMA抗体通过独特型-抗独特型相互作用与ivIg发生反应。自身免疫性甲状腺疾病患者的10份IgG制剂中有8份在与不同IgG:IgM摩尔比的自体IgM共同孵育时，AMA活性也受到抑制。这些观察结果表明，ivIg可通过独特型-抗独特型相互作用抑制抗微粒体抗体，并且这种相互作用在体内与IgM抗独特型抗体发生，为独特型网络调节在甲状腺自身免疫控制中的作用提供了证据。
