Michel U, McMaster J W, Findlay J K
Prince Henry's Institute of Medical Research, Clayton, Victoria, Australia.
J Mol Endocrinol. 1992 Oct;9(2):147-56. doi: 10.1677/jme.0.0090147.
The regulation of steady-state follistatin mRNA levels by different pituitary hormones and peptide factors was examined in granulosa cell cultures derived from diethylstilboestrol-treated immature rats. Cytosolic RNA from cell cultures was prepared by lysis and equal amounts of RNA from all samples were analysed with a solution-hybridization assay using a 32P-labelled antisense probe corresponding to a part of exon 5 together with a part of the 5' end of exon 6 of the rat follistatin gene. In addition, a specific 35S-labelled probe for cyclophilin was used as an internal standard. The results show that 5 micrograms FSH/l for 24 to 72 h stimulated steady-state follistatin mRNA levels, reaching levels 18.5-fold higher than controls. LH (0.2-100 micrograms/l) had only minor effects on follistatin mRNA levels in FSH-primed granulosa cells and prolactin, GH and IGF-I did not show any significant effects. Activin raised basal as well as FSH-stimulated steady-state follistatin mRNA levels up to ten- and twofold above controls respectively, whereas epidermal growth factor was found to inhibit FSH-stimulated follistatin mRNA levels in a dose-dependent manner. It is concluded that follistatin mRNA levels in granulosa cells are regulated by FSH rather than LH, and that the stimulation by FSH can be inhibited by epidermal growth factor but enhanced by activin. Activin alone was also capable of stimulating follistatin mRNA.
在己烯雌酚处理的未成熟大鼠来源的颗粒细胞培养物中，研究了不同垂体激素和肽因子对稳态卵泡抑素mRNA水平的调节。通过裂解制备细胞培养物的胞质RNA，使用与大鼠卵泡抑素基因外显子5的一部分以及外显子6的5'端的一部分相对应的32P标记反义探针，通过溶液杂交测定法分析所有样品等量的RNA。此外，使用亲环蛋白的特异性35S标记探针作为内标。结果表明，5μg FSH/l作用24至72小时可刺激稳态卵泡抑素mRNA水平，达到比对照高18.5倍的水平。LH（0.2 - 100μg/l）对FSH预处理的颗粒细胞中的卵泡抑素mRNA水平只有轻微影响，催乳素、生长激素和IGF - I未显示任何显著影响。激活素使基础以及FSH刺激的稳态卵泡抑素mRNA水平分别比对照高出10倍和2倍，而发现表皮生长因子以剂量依赖的方式抑制FSH刺激的卵泡抑素mRNA水平。得出结论，颗粒细胞中的卵泡抑素mRNA水平受FSH而非LH调节，并且FSH的刺激可被表皮生长因子抑制，但被激活素增强。单独的激活素也能够刺激卵泡抑素mRNA。
